Recombinant Anti-APOF antibody [EPR2908] - BSA and Azide free (ab232035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2908] to APOF - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-APOF antibody [EPR2908] - BSA and Azide free
See all APOF primary antibodies -
Description
Rabbit monoclonal [EPR2908] to APOF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HDL and Human plasma lysate
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General notes
ab232035 is the carrier-free version of ab92301.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2908 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
Target
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Function
Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 319 Human
- Omim: 107760 Human
- SwissProt: Q13790 Human
- Unigene: 534302 Human
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Alternative names
- Apo-F antibody
- Apof antibody
- APOF_HUMAN antibody
see all
Images
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All lanes : Anti-APOF antibody [EPR2908] (ab92301) at 1/2000 dilution
Lane 1 : HDL lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDaThis WB data was generated using the same anti-Apolipoprotein F antibody clone, EPR2908, in a different buffer formulation (cat# ab92301).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232035 has not yet been referenced specifically in any publications.