Anti-APOL2 antibody (ab196771)
Key features and details
- Rabbit polyclonal to APOL2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APOL2 antibody -
Description
Rabbit polyclonal to APOL2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human APOL2 (internal sequence).
Database link: Q9BQE5 -
Positive control
- WB: Extracts from COLO cells and HUVEC cells. ICC/IF: MCF7 cells. IHC-P: Human cervix carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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ICC/IF |
1/100 - 1/500.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/100 - 1/500. |
Target
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Function
May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles. -
Tissue specificity
Widely expressed; the highest levels are found in lung, thymus, pancreas, placenta, adult brain and prostate; also detected in spleen, liver, kidney, colon, small intestine, uterus, spinal cord, adrenal gland, salivary gland, trachea, mammary gland, skeletal muscle, testis and fetal brain and liver. -
Sequence similarities
Belongs to the apolipoprotein L family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23780 Human
- Omim: 607252 Human
- SwissProt: Q9BQE5 Human
- Unigene: 474740 Human
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Alternative names
- 9830006J20Rik antibody
- ApoL-II antibody
- APOL2 antibody
see all
Images
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All lanes : Anti-APOL2 antibody (ab196771) at 1/500 dilution
Lane 1 : COLO cell extracts
Lane 2 : HUVEC cell extracts
Lane 3 : HUVEC cell extracts with synthesized peptide
Predicted band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling APOL2 with ab196771 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
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Immunofluorescent analysis of MCF7 cells labeling APOL2 with ab196771 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196771 has been referenced in 1 publication.
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468