Overview

  • Product name

  • Description

    Rabbit polyclonal to APOL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human APOL2 aa 287-337. The exact sequence is proprietary.
    Database link: Q9BQE5

  • Positive control

    • WB: H226, HeLa, HEK-293T and jurkat whole cell lysate. IP: NCI-H226 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to APOL2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 3-6 µg/mg of lysate.
WB 1/1000. Predicted molecular weight: 37 kDa.

Target

  • Function

    May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.
  • Tissue specificity

    Widely expressed; the highest levels are found in lung, thymus, pancreas, placenta, adult brain and prostate; also detected in spleen, liver, kidney, colon, small intestine, uterus, spinal cord, adrenal gland, salivary gland, trachea, mammary gland, skeletal muscle, testis and fetal brain and liver.
  • Sequence similarities

    Belongs to the apolipoprotein L family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9830006J20Rik antibody
    • ApoL-II antibody
    • APOL2 antibody
    • APOL2_HUMAN antibody
    • APOL3 antibody
    • Apol8 antibody
    • ApoLII antibody
    • Apolipoprotein L 2 antibody
    • Apolipoprotein L, 2 antibody
    • Apolipoprotein L-II antibody
    • Apolipoprotein L2 antibody
    • Apolipoprotein LII antibody
    • ApolipoproteinL2 antibody
    see all

Images

  • All lanes : Anti-APOL2 antibody (ab241378) at 1/1000 dilution

    Lane 1 : NCI-H226 whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 37 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • APOL2 was immunoprecipitated from NCI-H226 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241378 at 8 µL per reaction. Western bot was performed on the immunoprecipitate using ab241378 at 1/1000 dilution.

    Lane 1: ab241378 IP in NCI-H226 whole cell lysate.
    Lane 2: Control IgG IP inNCI-H226 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

    Lysates prepared in NETN buffer.

     

     

     

     

References

ab241378 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241378.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up