Recombinant Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-347181-anti-apolipoprotein-a-I-antibody-ep1368y-westernblot-hepg2-human.jpg "Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
- Suitable for: Flow Cyt (Intra), Sandwich ELISA, WB, IP, IHC-P, ICC/IF, ELISA
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Apolipoprotein A I antibody [EP1368Y]
See all Apolipoprotein A I primary antibodies -
Description
Rabbit monoclonal [EP1368Y] to Apolipoprotein A I -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), Sandwich ELISA, WB, IP, IHC-P, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human Apolipoprotein A I aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Human liver and fetal lysates IHC-P: Human liver Tissue ICC/IF: HepG2 cells Flow Cyt (intra): HepG2 cells IP: HepG2 cell lysates, Human fetal liver tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1368Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Apolipoprotein A I antibody [EP1368Y] - Low endotoxin, Azide free (ab185132)
- Anti-Apolipoprotein A I antibody [EP1368Y] - BSA and Azide free (ab271849)
- Alexa Fluor® 488 Anti-Apolipoprotein A I antibody [EP1368Y] (ab279676)
- Alexa Fluor® 647 Anti-Apolipoprotein A I antibody [EP1368Y] (ab279677)
- PE Anti-Apolipoprotein A I antibody [EP1368Y] (ab282174)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
|
| Sandwich ELISA |
Use a concentration of 0 - 0.1 mg/ml. Can be paired for Sandwich ELISA with HRP Goat polyclonal to Apolipoprotein A I (ab20784).
|
|
| WB | (5) |
1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
For unpurified use at 1/20000 dilution. |
| IP |
1/60.
|
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF | (2) |
1/100 - 1/250.
|
| ELISA |
Use at an assay dependent concentration.
Antibody conc. = 0 - 1000 ng/ml |
| Notes |
|---|
|
Flow Cyt (Intra)
1/20. |
|
Sandwich ELISA
Use a concentration of 0 - 0.1 mg/ml. Can be paired for Sandwich ELISA with HRP Goat polyclonal to Apolipoprotein A I (ab20784). |
|
WB
1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). For unpurified use at 1/20000 dilution. |
|
IP
1/60. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
|
ELISA
Use at an assay dependent concentration. Antibody conc. = 0 - 1000 ng/ml |
Target
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Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. -
Tissue specificity
Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. -
Involvement in disease
Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Belongs to the apolipoprotein A1/A4/E family. -
Post-translational
modificationsPalmitoylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 335 Human
- Omim: 107680 Human
- SwissProt: P02647 Human
- Unigene: 93194 Human
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Alternative names
- Apo-AI antibody
- ApoA I antibody
- ApoA-I antibody
see all
Images
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Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-347180-anti-apolipoprotein-a-I-antibody-ep1368y-westernblot-liver-human.jpg)
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + Human liver lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
![Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-65467-anti-apolipoprotein-a-i-antibody-ep1368y-western-blot.gif)
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution ((unpurified)) + fetal liver lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-6-anti-apolipoprotein-a-I-antibody-ep1368y-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Apolipoprotein A I with purified ab52945 at 1/100 dilution (1.95 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-7-anti-apolipoprotein-a-I-antibody-ep1368y-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/250 dilution (0.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-8-anti-apolipoprotein-a-I-antibody-ep1368y-flowcytometry-hepg2-human.jpg)
Flow Cytometry (Intracellular) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-9-anti-apolipoprotein-a-I-antibody-ep1368y-immunoprecipitation-fetal-liver-human.jpg)
Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)ab52945 (purified) at 1/20 dilution (1ug) immunoprecipitating Apolipoprotein A I in Human fetal liver lysates.
Lane 1: Human fetal liver lysates 10ug
Lane 2 (+): ab52945 & Human fetal liver lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52945 in Human fetal liver lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-445216-AntiApolipoprotein-A-I-antibody-EP1368Y-Human.jpg)
ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)ELISA analysis of Apolipoprotein A I recombinant protein at 1000 ng/mL with ab52945 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-346998-anti-apolipoprotein-a-I-antibody-ep1368y-elisa.png)
ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)Direct ELISA antigen dose-response curve using purified ab52945 at 0-1000ng/mL. Antigen (apo AI peptide) concentration of 1000ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-65466-anti-apolipoprotein-a-i-antibody-ep1368y-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
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![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-65465-anti-apolipoprotein-a-i-antibody-ep1368y-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.
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![Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)](https://www.abcam.com/ps/products/52/ab52945/Images/ab52945-13-benefits-of-recombinant-antibodies.png)
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab52945 has been referenced in 24 publications.
- Begue F et al. Altered high-density lipoprotein composition and functions during severe COVID-19. Sci Rep 11:2291 (2021). PubMed: 33504824
- Jacobs-Cachá C et al. A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation. J Clin Med 10:N/A (2021). PubMed: 34069888
- Wu B et al. Separation and characterization of extracellular vesicles from human plasma by asymmetrical flow field-flow fractionation. Anal Chim Acta 1127:234-245 (2020). PubMed: 32800129
- Kim SS et al. Serum small extracellular vesicle-derived LINC00853 as a novel diagnostic marker for early hepatocellular carcinoma. Mol Oncol 14:2646-2659 (2020). PubMed: 32525601
- Karvinen S et al. MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise. Front Physiol 11:676 (2020). PubMed: 32760282