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RecombinantRabMAb

Recombinant Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)

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Western blot - Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)
  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)
  • Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPSISR27] to Apolipoprotein A I
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Apolipoprotein A I antibody [EPSISR27]
    See all Apolipoprotein A I primary antibodies
  • Description

    Rabbit monoclonal [EPSISR27] to Apolipoprotein A I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Apolipoprotein A I. The exact sequence is proprietary.
    Database link: P02647

  • Positive control

    • Human small intestine and plasma lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPSISR27
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease
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Associated products

  • Alternative Versions

    • Anti-Apolipoprotein A I antibody [EPSISR27] - BSA and Azide free (ab232048)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein A I (ab50239)
  • Related Products

    • Recombinant Human Apolipoprotein A I (ab50239)
    • Native Human Apolipoprotein A I (ab90760)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
    • Tissue specificity

      Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
    • Involvement in disease

      Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
      Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
      Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
      Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • Sequence similarities

      Belongs to the apolipoprotein A1/A4/E family.
    • Post-translational
      modifications

      Palmitoylated.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02647 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 335 Human
      • Omim: 107680 Human
      • SwissProt: P02647 Human
      • Unigene: 93194 Human
      • Alternative names

        • Apo-AI antibody
        • ApoA I antibody
        • ApoA-I antibody
        • APOA1 antibody
        • APOA1_HUMAN antibody
        • Apolipoprotein A-I(1-242) antibody
        • Apolipoprotein A1 antibody
        • Apolipoprotein AI antibody
        • Apolipoprotein of high density lipoprotein antibody
        • ApolipoproteinAI antibody
        • Brp14 antibody
        • high density lipoprotein uptake antibody
        • Ltw1 antibody
        • Lvtw1 antibody
        • MGC117399 antibody
        • Sep1 antibody
        • Sep2 antibody
        see all

      Images

      • Western blot - Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)
        Western blot - Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)
        All lanes : Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710) at 1/1000 dilution

        Lane 1 : Human small intestine lysate
        Lane 2 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 30 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)
        Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)

        Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Apolipoprotein A I with ab151710 at 1/250 dilution.

      • Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)
        Anti-Apolipoprotein A I antibody [EPSISR27] (ab151710)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab151710? Please let us know so that we can cite the reference in this datasheet.

      ab151710 has not yet been referenced specifically in any publications.

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