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Rabbit monoclonal [EPR2913] to Apolipoprotein A II
ICC/IF more details Unsuitable for:
IHC-P or IP
Synthetic peptide within Human Apolipoprotein A II aa 50-150. The exact sequence is proprietary.
Human plasma lysate. HEPG2 cells; rat primary astrocytes,
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/500.
Is unsuitable for IHC-P or IP.
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
Plasma; synthesized in the liver and intestine.
Belongs to the apolipoprotein A2 family.
Phosphorylation sites are present in the extracelllular medium.
Information by UniProt
APO A2 antibody
Apo AII antibody
Western blot - Anti-Apolipoprotein A II antibody [EPR2913] (ab92478)
Anti-Apolipoprotein A II antibody [EPR2913] (ab92478) at 1/1000 dilution + Human plasma
lysate at 10 µg Secondary HRP labelled goat anti-rabbit antibody at 1/2000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II antibody [EPR2913] (ab92478) This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury
Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A II antibody [EPR2913] (ab92478)
Ab92478 at 1/100 dilution staining Apolipoprotein A II in HepG2 cells by immunofluorescence.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"