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    apolipoprotein-a-iiapoa-ii-antibody-ab76651.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Anti-Apolipoprotein A II/ApoA-II antibody (ab76651)

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Western blot - Anti-Apolipoprotein A II/ApoA-II antibody (ab76651)

    Key features and details

    • Rabbit polyclonal to Apolipoprotein A II/ApoA-II
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (ab92478)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Apolipoprotein A II/ApoA-II antibody
      See all Apolipoprotein A II/ApoA-II primary antibodies
    • Description

      Rabbit polyclonal to Apolipoprotein A II/ApoA-II
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee, Rhesus monkey
    • Immunogen

      Synthetic peptide corresponding to Human Apolipoprotein A II/ApoA-II aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab87373)

    • Positive control

      • This antibody gave a positive signal in Human Plasma Total Protein Lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipid transport
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Apolipoprotein A II/ApoA-II (ab192286)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab76651 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

    Target

    • Function

      May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
    • Tissue specificity

      Plasma; synthesized in the liver and intestine.
    • Sequence similarities

      Belongs to the apolipoprotein A2 family.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02652 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 336 Human
      • Omim: 107670 Human
      • SwissProt: Q8MIQ5 Chimpanzee
      • SwissProt: P02652 Human
      • Unigene: 237658 Human
      • Alternative names

        • APO A2 antibody
        • Apo AII antibody
        • Apo-AII antibody
        • APOA 2 antibody
        • ApoA II antibody
        • ApoA-II antibody
        • APOA2 antibody
        • APOA2_HUMAN antibody
        • APOAII antibody
        • Apolipoprotein A II antibody
        • Apolipoprotein A-II(1-76) antibody
        • Apolipoprotein A2 antibody
        • Apolipoprotein AII antibody
        • ApolipoproteinA II antibody
        • OTTHUMP00000032244 antibody
        see all

      Images

      • Western blot - Anti-Apolipoprotein A II/ApoA-II antibody (ab76651)
        Western blot - Anti-Apolipoprotein A II/ApoA-II antibody (ab76651)
        Anti-Apolipoprotein A II/ApoA-II antibody (ab76651) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 11 kDa
        Observed band size: 11 kDa


        Exposure time: 14 minutes

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab76651? Please let us know so that we can cite the reference in this datasheet.

      ab76651 has not yet been referenced specifically in any publications.

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