Overview

  • Product name

    Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913]
    See all Apolipoprotein A II/ApoA-II primary antibodies
  • Description

    Rabbit monoclonal [EPR2913] to Apolipoprotein A II/ApoA-II
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Apolipoprotein A II/ApoA-II aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human plasma lysate. HEPG2 cells; rat primary astrocytes,
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Apolipoprotein A II

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab92478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
    • Tissue specificity

      Plasma; synthesized in the liver and intestine.
    • Sequence similarities

      Belongs to the apolipoprotein A2 family.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • APO A2 antibody
      • Apo AII antibody
      • Apo-AII antibody
      • APOA 2 antibody
      • ApoA II antibody
      • ApoA-II antibody
      • APOA2 antibody
      • APOA2_HUMAN antibody
      • APOAII antibody
      • Apolipoprotein A II antibody
      • Apolipoprotein A-II(1-76) antibody
      • Apolipoprotein A2 antibody
      • Apolipoprotein AII antibody
      • ApolipoproteinA II antibody
      • OTTHUMP00000032244 antibody
      see all

    Images

    • Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (ab92478) at 1/1000 dilution + Human plasma
      lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 11 kDa
      Observed band size: 11 kDa

    • Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II/ApoA-II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody. 

      See Abreview

    • Ab92478 at 1/100 dilution staining Apolipoprotein A II/ApoA-II in HepG2 cells by immunofluorescence.

    References

    This product has been referenced in:

    • Zhao L  et al. PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer. Biomed Pharmacother 118:109251 (2019). Read more (PubMed: 31351426) »
    • Holzer M  et al. HDL structure and function is profoundly affected when stored frozen in the absence of cryoprotectants. J Lipid Res 58:2220-2228 (2017). Read more (PubMed: 28893842) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary astrocyte culture)
    Permeabilization
    Yes - 0.1M PBS with 1% Triton X
    Specification
    Primary astrocyte culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Methanol

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 06 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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