Anti-Apolipoprotein A V/APOA5 antibody (ab77358)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Apolipoprotein A V/APOA5 antibody
See all Apolipoprotein A V/APOA5 primary antibodies -
Description
Goat polyclonal to Apolipoprotein A V/APOA5 -
Host species
Goat -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon
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Immunogen
Synthetic peptide corresponding to Human Apolipoprotein A V/APOA5 (C terminal).
Sequence:HSLHDQGHSHLGDP
Database link: NP_443200.2
(Peptide available asab78151) -
Positive control
- Human liver lysates
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General notes
This product was previously labelled as Apolipoprotein A V
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab77358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| WB |
Application notes
Peptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 41 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 41 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. -
Tissue specificity
Liver and plasma. -
Involvement in disease
Defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR)[MIM:145750].mFamilial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.
Defects in APOA5 are a cause of hyperlipoproteinemia type 5 (HLPP5) [MIM:144650]. HLPP5 is characterized by increased amounts of chylomicrons and very low density lipoprotein (VLDL) and decreased low density lipoprotein (LDL) and high density lipoprotein (HDL) in the plasma after a fast. Numerous conditions cause this phenotype, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A (GSD1A) [MIM:232200]. -
Sequence similarities
Belongs to the apolipoprotein A1/A4/E family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 116519 Human
- Omim: 606368 Human
- SwissProt: Q6Q788 Human
- Unigene: 283923 Human
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Alternative names
- Apo-AV antibody
- ApoA-V antibody
- Apoa5 antibody
see all
Images
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Western blot - Anti-Apolipoprotein A V/APOA5 antibody (ab77358)Anti-Apolipoprotein A V/APOA5 antibody (ab77358) at 0.3 µg/ml + human liver lysate in RIPA buffer at 35 µg
Predicted band size: 41 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab77358 has not yet been referenced specifically in any publications.
