Key features and details
- Mouse monoclonal [C6] to Apolipoprotein A V/APOA5
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-Apolipoprotein A V/APOA5 antibody [C6]
See all Apolipoprotein A V/APOA5 primary antibodies
DescriptionMouse monoclonal [C6] to Apolipoprotein A V/APOA5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Recombinant fragment corresponding to Mouse Apolipoprotein A V/APOA5 aa 21-210. Two N-terminal Tags, His-tag and GST-tag (Expresses in E.coli).
RKSLWDYFSQNSWSKGVMGQPQKLAQENLKGSFEQDLYNMNNYLEKLGPL RGPGKEPPLLAQDPEGIRKQLQQELGEVSSRLEPYMAAKHQQVGWNLEGL RQQLKPYTAELMEQVGLSVQELQEQLRVVGEDTKAQLLGGVDEALNLLQD MQSRVLHHTDRVKELFHPYAERLVTGIGHHVQELHRSVAP
Database link: Q8C7G5
- WB: Recombinant Mouse Apolipoprotein A V/APOA5 protein; Mouse liver tissue lysate. IHC-P: Mouse liver tissue.
This product was previously labelled as Apolipoprotein A V
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab239579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml.|
|IHC-P||Use a concentration of 5 - 30 µg/ml.|
FunctionMinor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.
Tissue specificityLiver and plasma.
Involvement in diseaseDefects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR)[MIM:145750].mFamilial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.
Defects in APOA5 are a cause of hyperlipoproteinemia type 5 (HLPP5) [MIM:144650]. HLPP5 is characterized by increased amounts of chylomicrons and very low density lipoprotein (VLDL) and decreased low density lipoprotein (LDL) and high density lipoprotein (HDL) in the plasma after a fast. Numerous conditions cause this phenotype, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A (GSD1A) [MIM:232200].
Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- Apo-AV antibody
- ApoA-V antibody
- Apoa5 antibody
Paraffin-embedded mouse liver tissue stained for Apolipoprotein A V/APOA5 using ab239579 at 30 μg/mL in immunohistochemical analysis. DAB staining.
Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579) at 1 µg/ml + Mouse liver tissue lysate
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579) at 5 µg/ml + Recombinant Mouse Apolipoprotein A V/APOA5 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239579 has not yet been referenced specifically in any publications.