Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579)
Key features and details
- Mouse monoclonal [C6] to Apolipoprotein A V/APOA5
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Apolipoprotein A V/APOA5 antibody [C6]
See all Apolipoprotein A V/APOA5 primary antibodies -
Description
Mouse monoclonal [C6] to Apolipoprotein A V/APOA5 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse Apolipoprotein A V/APOA5 aa 1-250. Two N-terminal Tags, His-tag and GST-tag (Expresses in E.coli).
Database link: Q8C7G5 -
Positive control
- WB: Recombinant Mouse Apolipoprotein A V/APOA5 protein; Mouse liver tissue lysate. IHC-P: Mouse liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 - 30 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. |
IHC-P
Use a concentration of 5 - 30 µg/ml. |
Target
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Function
Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. -
Tissue specificity
Liver and plasma. -
Involvement in disease
Defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR)[MIM:145750].mFamilial hypertriglyceridemia is a common inherited disorder in which the concentration of very low density lipoprotein (VLDL) is elevated in the plasma. This leads to increased risk of heart disease, obesity, and pancreatitis.
Defects in APOA5 are a cause of hyperlipoproteinemia type 5 (HLPP5) [MIM:144650]. HLPP5 is characterized by increased amounts of chylomicrons and very low density lipoprotein (VLDL) and decreased low density lipoprotein (LDL) and high density lipoprotein (HDL) in the plasma after a fast. Numerous conditions cause this phenotype, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A (GSD1A) [MIM:232200]. -
Sequence similarities
Belongs to the apolipoprotein A1/A4/E family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 66113 Mouse
- SwissProt: Q8C7G5 Mouse
- Unigene: 29738 Mouse
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Alternative names
- Apo-AV antibody
- ApoA-V antibody
- Apoa5 antibody
see all
Images
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Paraffin-embedded mouse liver tissue stained for Apolipoprotein A V/APOA5 using ab239579 at 30 μg/mL in immunohistochemical analysis. DAB staining.
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Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579) at 1 µg/ml + Mouse liver tissue lysate
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution -
Anti-Apolipoprotein A V/APOA5 antibody [C6] (ab239579) at 5 µg/ml + Recombinant Mouse Apolipoprotein A V/APOA5 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab239579 has been referenced in 1 publication.
- Yu Y et al. Exercise Alleviates the Apolipoprotein A5-Toll-Like Receptor 4 Axis Impairment in Mice With High-Fat Diet-Induced Non-alcoholic Steatohepatitis. Front Physiol 12:783341 (2021). PubMed: 35035359