Overview

  • Product name

    Anti-Apolipoprotein B antibody [7B8]
    See all Apolipoprotein B primary antibodies
  • Description

    Mouse monoclonal [7B8] to Apolipoprotein B
  • Host species

    Mouse
  • Specificity

    Ab39560 has specificity for Apolipoprotein B from serum/plasma. No crossreactivity is seen with Apolipoprotein A1.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human LDL.

Properties

Applications

Our Abpromise guarantee covers the use of ab39560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Ab39560 reacts with Apolipoprotein B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against Apolipoprotein B, and also with isolated LDL directly coated onto the microtiter plate.
WB Use at an assay dependent concentration. Predicted molecular weight: 260 kDa. Ab39560 binds to Apolipoprotein B in reduced as well as in non-reduced forms, using isolated LDL as antigen.
IHC-P 1/100 - 1/400.

Target

  • Function

    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in disease

    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similarities

    Contains 1 vitellogenin domain.
  • Post-translational
    modifications

    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apo B 100 antibody
    • Apo B antibody
    • Apo B-100 antibody
    • Apo B-48 antibody
    • Apo B100 antibody
    • Apo B48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B (including Ag(x) antigen) antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B 48 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B100 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/100. Signal is strong in the veins of the glomerulus.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/400. Plasma of the veins was stained strongly.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/400. Plasma veins were stained strongly.

References

This product has been referenced in:

  • Seckler HDS  et al. A Targeted, Differential Top-Down Proteomic Methodology for Comparison of ApoA-I Proteoforms in Individuals with High and Low HDL Efflux Capacity. J Proteome Res 17:2156-2164 (2018). Read more (PubMed: 29649363) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your patience.

I now heard back form the lab and they confirmed that was never experimentally tested if ab39560 also binds to apoB48 alone.

As stated on the datasheet ab39560 reacts with Apolipoprotein B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against Apolipoprotein B, and also with isolated LDL (apo B-100) directly coated onto the microtiter plate.

I am sorry I could not answer your question on this occasion and hope this information is nevertheless helpful.

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