Anti-Apolipoprotein B antibody [7B8] (ab39560)
Key features and details
- Mouse monoclonal [7B8] to Apolipoprotein B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Apolipoprotein B antibody [7B8]
See all Apolipoprotein B primary antibodies -
Description
Mouse monoclonal [7B8] to Apolipoprotein B -
Host species
Mouse -
Specificity
Ab39560 has specificity for Apolipoprotein B from serum/plasma. No crossreactivity is seen with Apolipoprotein A1. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human LDL.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7B8 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/400.
|
Notes |
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IHC-P
1/100 - 1/400. |
Target
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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Alternative names
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/100. Signal is strong in the veins of the glomerulus.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/400. Plasma of the veins was stained strongly.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/400. Plasma veins were stained strongly.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab39560 has been referenced in 2 publications.
- Arai C et al. Trehalose itself plays a critical role on lipid metabolism: Trehalose increases jejunum cytoplasmic lipid droplets which negatively correlated with mesenteric adipocyte size in both HFD-fed trehalase KO and WT mice. Nutr Metab (Lond) 17:22 (2020). PubMed: 32206077
- Seckler HDS et al. A Targeted, Differential Top-Down Proteomic Methodology for Comparison of ApoA-I Proteoforms in Individuals with High and Low HDL Efflux Capacity. J Proteome Res 17:2156-2164 (2018). PubMed: 29649363