- Datasheet
- Specific References (29)
- Protocols
Overview
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Product nameAnti-Apolipoprotein B antibody
See all Apolipoprotein B primary antibodies -
DescriptionRabbit polyclonal to Apolipoprotein B
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Host speciesRabbit
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SpecificityCross-reacts with Apo B48 and B100.
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Tested applicationsSuitable for: IHC-Fr, IP, ELISA, IHC-P, Immunodiffusion, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Full length native protein (purified) corresponding to Mouse Apolipoprotein B. Apolipoprotein B48 purified from pooled mouse plasma very low-density lipoprotein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum -
PurityWhole antiserum
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Purification notesHeat inactivated. Product is 0.22um filtered.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab20737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/1000. | |
| IP | Use at an assay dependent concentration. PubMed: 18497885 | |
| ELISA | Use at an assay dependent concentration. PubMed: 21668821 | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Immunodiffusion | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 549 kDa. |
Target
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FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
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Involvement in diseaseHypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similaritiesContains 1 vitellogenin domain.
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Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 338 Human
- Entrez Gene: 238055 Mouse
- Omim: 107730 Human
- SwissProt: P04114 Human
- SwissProt: E9Q414 Mouse
- Unigene: 120759 Human
- Unigene: 221239 Mouse
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Alternative names
- Apo B-100 antibody
- Apo B-48 antibody
- ApoB 100 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein B antibody (ab20737)Image courtesy of an anonymous Abreview.ab20737 staining Apoplipoprotein B in murine intestinal tissue by Immunohistochemistry (frozen sections). Tissue was fixed with 10% formalin and permeabilized using Triton X-100. Samples were then blocked with 10% serum for 30 minutes at 24°C followed by incubation with the primary antibody, at a 1/100 dilution, for 16 hours at 4°C. A donkey anti-rabbit F(ab')2 fragment conjugated to Cy5® was used as secondary antibody at a 1/1000 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (ab20737)Image courtesy of Ryusuke Takechi by Abreview.ab20737 at a 1/100 dilution staining Apolipoprotein B in murine small intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed. Samples were then permeabilized using 0.2% PBST and blocked using 20% serum. An Alexa Fluor® 488 conjugated Goat anti-rabbit IgG was used as the secondary at a 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (ab20737)This image is courtesy of an anonymous Abreviewab20737 staining Apolipoprotein in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% neutral buffered formalin and blocked with 1.5% serum for 45 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (8.5ug/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (7ug/ml) was used as the secondary antibody.
References
This product has been referenced in:
- Dong Z et al. Loss of LCAT activity in the golden Syrian hamster elicits pro-atherogenic dyslipidemia and enhanced atherosclerosis. Metabolism 83:245-255 (2018). WB . Read more (PubMed: 29526535) »
- Thänert R et al. Host-inherent variability influences the transcriptional response of Staphylococcus aureus during in vivo infection. Nat Commun 8:14268 (2017). Read more (PubMed: 28155859) »
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