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  1. Link

    apolipoprotein-b-antibody-ab20737.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Anti-Apolipoprotein B antibody (ab20737)

  • Datasheet
  • SDS
Reviews (6)Q&A (21)References (65)

Product price, shipping and contact information

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Abpromise

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Find out more.

Key features and details

  • Rabbit polyclonal to Apolipoprotein B
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Apolipoprotein B antibody
    See all Apolipoprotein B primary antibodies
  • Description

    Rabbit polyclonal to Apolipoprotein B
  • Host species

    Rabbit
  • Specificity

    Cross-reacts with Apo B48 and B100.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Mouse Apolipoprotein B. Apolipoprotein B48 purified from pooled mouse plasma very low-density lipoprotein.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Purification notes

    Heat inactivated. Product is 0.22um filtered.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Fibrinolysis
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein B (ab80037)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500. Predicted molecular weight: 515 kDa.
Notes
WB
1/500. Predicted molecular weight: 515 kDa.

Target

  • Function

    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in disease

    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similarities

    Contains 1 vitellogenin domain.
  • Post-translational
    modifications

    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localization

    Cytoplasm. Secreted.
  • Target information above from: UniProt accession P04114 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 338 Human
    • Entrez Gene: 238055 Mouse
    • Omim: 107730 Human
    • SwissProt: P04114 Human
    • SwissProt: E9Q414 Mouse
    • Unigene: 120759 Human
    • Unigene: 221239 Mouse
    • Alternative names

      • Apo B 100 antibody
      • Apo B antibody
      • Apo B-100 antibody
      • Apo B-48 antibody
      • Apo B100 antibody
      • Apo B48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B (including Ag(x) antigen) antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B 48 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B100 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (65)

    Publishing research using ab20737? Please let us know so that we can cite the reference in this datasheet.

    ab20737 has been referenced in 65 publications.

    • Balbi C  et al. Circulating extracellular vesicles are endowed with enhanced procoagulant activity in SARS-CoV-2 infection. EBioMedicine 67:103369 (2021). PubMed: 33971404
    • Zhang Y  et al. Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1. Nat Commun 12:1731 (2021). PubMed: 33741962
    • Qin Y  et al. Phosphatidylinositol-(4,5)-Bisphosphate Regulates Plasma Cholesterol Through LDL (Low-Density Lipoprotein) Receptor Lysosomal Degradation. Arterioscler Thromb Vasc Biol 40:1311-1324 (2020). PubMed: 32188273
    • Chen PY  et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms. Cell Stem Cell 26:542-557.e11 (2020). PubMed: 32243809
    • Kim JT  et al. A Plasma Protein Network Regulates PM20D1 and N-Acyl Amino Acid Bioactivity. Cell Chem Biol 27:1130-1139.e4 (2020). PubMed: 32402239
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 27 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-Apolipoprotein B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Rat Cell lysate - whole cell (McA-7777 Rat hepatoma cell line)
    Total protein in input
    500000 cells
    Immuno-precipitation step
    Protein A
    Specification
    McA-7777 Rat hepatoma cell line
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Mikael Rutberg

    Verified customer

    Submitted Mar 01 2019

    Western blot abreview for Anti-Apolipoprotein B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Whole liver)
    Gel Running Conditions
    Reduced Denaturing (5% gel, run at 150V for 1.5 hours.)
    Loading amount
    40 µg
    Specification
    Whole liver
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 20 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    10% neutral buffered formalin
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0 (20 min, 93*C)
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 24 2013

    Question

    Send WB protocol for large proteins (550 kD) and any specifics for WB protocol with ab20737 (ApoB)

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2014

    Answer


    Below is information regarding the use of ab20737 in western blot.

    1. Working dilution for immunoblotting - start low 1:100 - 1:500.
    2. Molecular Weight of Apo B100 is 500kDa; Apo B48 is 250kDa. The western blot was run against purified LDL from mouse and against human plasma. Also tested mouse VLDL to detect ApoB48. When testing whole plasma, you may see additional bands (possible proteolytic fragments of ApoB100 and B48).
    3. Use 1% BSA for blocking in the immunoblot part of the procedure.

    Our general recommendations for transfer of large proteins, and a recommended paper that outlines a protocol for large protein transfer is given in our western blot guide below. As I mentioned, I recommended ensuring there is no methanol in your transfer buffer and including 0.1% SDS in the transfer buffer as well.

    https://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

    Below is a link to a paper that used ab20737 in western blot with mouse samples.

    http://www.ncbi.nlm.nih.gov/pubmed/17438339?dopt=Abstract

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Jan 10 2014

    Question

    We are interested in this antibody. Can we get an aliquot so that we can test in our system and if it works then we will place the order and submit the abreview.

    Do let me know the possibility.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2013

    Answer

    Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If a species or application is untested you can use our products without financial risk by participating in our AbTrial program.

    You would have to buy the ab117317 but once you submitted the Abreview you will receive a credit over the whole value of ab117317. You will receive this for either a positive and negative result of the testing.

    I hope this information is helpful. Please let me know if you like to except the AbTrial offer and I would be happy to issue the Trial Code for you. If you have further questions please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jan 09 2013

    Question

    Inquiry: I am looking for antibody against apolipoprotein B-100 which can recognize human, mouse and rat. Does Ab20737 recognize rat as well. Or do suggest me antibody which can cross react with rat, mouse and human. We require the antibody for western as well as ICC. Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 16 2012

    Answer

    Thank you for your inquiry.

    I am happy to confirm that we offer an Anti-Apolipoprotein B antibody that is tested and guaranteed for ICC and WB in rat, human and mouse samples:

    ab117317b (Anti-Apolipoprotein B antibody)

    https://www.abcam.com/Apolipoprotein-B-antibody-ab117317.html (or use the following: https://www.abcam.com/Apolipoprotein-B-antibody-ab117317.html).

    ab117317 is a chicken IgY antibody a suitbale secondary antibody is anti chicken IgY.

    For example:

    ab97135

    https://www.abcam.com/Goat-Chicken-IgY-HL-HRP-ab97135.html (or use the following: https://www.abcam.com/Goat-Chicken-IgY-HL-HRP-ab97135.html).

    Apolipoprotein B is a very big protein (over 500kDa plus many modifications) and I would suggest to review these tips for big proteins in WB.

    The following reference discusses a gel and buffer system that allows transfer of proteins as large as 500 kD:

    Bolt and Mahoney, High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate–polyacrylamide, gel electrophoresis. Analytical Biochemistry 247, 185–192 (1997).

    https://www.abcam.com/index.html?pageconfig=resource&rid=11404#C2

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

    Abcam Scientific Support

    Answered on Oct 16 2012

    Question

    The order reference number is xxx with invoice number xxx. The order was placed on xxx this year for the amount of $.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 09 2012

    Answer

    Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    Answered on Oct 09 2012

    Question

    Ok, in that case, can I get the credit back on my credit card?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 06 2012

    Answer

    Thank you for your reply.

    I can gladly create this credit note/refund for you provided that you have purchase this item within the last six months. In order for me to issue this could you please provide me the Abcam order confirmation number for purchase of ab20737.

    I look forward to your reply. Please let me know if you have any other questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 06 2012

    Question

    How does the credit work? How long will it be valid for?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2012

    Answer

    Thank you for your reply. If your Abcam order was placed by credit card, the credit note indicates the dollar amount that will be refunded to your credit card account. Please allow 2-5 business days for the credit to process and appear on your statement. If your Abcam order was placed by Purchase Order andthe original invoice has not yet been paid to Abcam, your credit note will be forwarded on to your institution’s accounts payable department. They will subtract the credit amount from the invoice total, and disburse a check (if any) for the correct amount payable to Abcam. If your Abcam order was placed by Purchase Order andthe original invoice has already been paid, your credit note will be sent to your accounts payable department, and is credited back against the PO used for the purchase and put back into your budget. If your budget has been closed and this is no longer possible, you can request a refund by check instead at us.credits@abcam.com. The credit can also serve as a reference for a discount on a future purchase. I hope you have found this information helpful. Please do not hesitate to contact us with any additional questions you may have.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2012

    Question

    We tried diluting your antibody to 1:25,000 and we still could not get it to work. We used your antibody to coat the wells (sandwich ELISA). As you can see in the attached file, whenever we use your antibody ("Rabbit" 1:25,000) in combination with other commercial antibodies we could not get a good standard curve. The absorbance did not get saturated so the 1:25,000 dilution should not be the issue. By the way, we used an HRP substrate kit to detect the signals. The other ApoB antibodies that we used were: mouse monoclonal. Both of these monoclonal antibodies were purchased from another company. When we use their antibodies as a combination, the assay works. Our recombinant ApoB (served as standards) was purchased from another company. We used your protocol to do our ELISA.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2012

    Answer

    Thank you for taking time to provide this information and for contacting us. I am sorry to hear this antibody is not providing satisfactory results and that these suggestions did not help.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I wish to apologize for the inconvenience. If you have purchase this item within the last six months I am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2012

    1-10 of 27 Abreviews or Q&A

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