Anti-Apolipoprotein B antibody (ab231574)
Key features and details
- Rabbit polyclonal to Apolipoprotein B
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Apolipoprotein B antibody
See all Apolipoprotein B primary antibodies -
Description
Rabbit polyclonal to Apolipoprotein B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Human Apolipoprotein B aa 3350-3550. (Expressed in E.coli).
Database link: P04114 -
Positive control
- WB: Recombinant human Apolipoprotein B protein; Pig liver lysate. IHC-P: Human liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231574 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 516 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 516 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 338 Human
- Entrez Gene: 100523371 Pig
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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Alternative names
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
Images
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Anti-Apolipoprotein B antibody (ab231574) at 2 µg/ml + Recombinant human Apolipoprotein B protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 516 kDa -
Anti-Apolipoprotein B antibody (ab231574) at 2 µg/ml + Pig liver lysate
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 516 kDa -
Formalin-fixed, paraffin-embedded human liver tissue stained for Apolipoprotein B using ab231574 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231574 has been referenced in 1 publication.
- Xu X et al. Apolipoprotein B Is Associated With the Microenvironment of Cholangiocarcinoma. Front Oncol 11:654689 (2021). PubMed: 33954113