For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    apolipoprotein-b-antibody-ab7616.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
Share by email

Anti-Apolipoprotein B antibody (ab7616)

  • Datasheet
  • SDS
Reviews (2)Q&A (10)References (13)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Apolipoprotein B antibody (ab7616)

    Key features and details

    • Goat polyclonal to Apolipoprotein B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    ELISA
    Human Anti-Chikungunya Virus IgM ELISA Kit (ab177848)
    Primary
    Product image
    Anti-G3BP antibody [EPR13986(B)] (ab181150)
    Protein
    Product image
    Recombinant Human Apolipoprotein B (ab80037)

    View more associated products

    Overview

    • Product name

      Anti-Apolipoprotein B antibody
      See all Apolipoprotein B primary antibodies
    • Description

      Goat polyclonal to Apolipoprotein B
    • Host species

      Goat
    • Specificity

      Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Full length native apoLipoprotein Type B (purified).

    • General notes

      This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

      At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 4.7625% Sodium borate, 0.146% EDTA, 0.435% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
    • Primary antibody notes

      At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Fibrinolysis
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human Apolipoprotein B (ab80037)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7616 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/200 - 1/1000.
    Notes
    WB
    1/200 - 1/1000.

    Target

    • Function

      Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • Involvement in disease

      Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • Sequence similarities

      Contains 1 vitellogenin domain.
    • Post-translational
      modifications

      Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • Cellular localization

      Cytoplasm. Secreted.
    • Target information above from: UniProt accession P04114 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 338 Human
      • Omim: 107730 Human
      • SwissProt: P04114 Human
      • Unigene: 120759 Human
      • Alternative names

        • Apo B 100 antibody
        • Apo B antibody
        • Apo B-100 antibody
        • Apo B-48 antibody
        • Apo B100 antibody
        • Apo B48 antibody
        • ApoB 100 antibody
        • ApoB 48 antibody
        • APOB antibody
        • APOB_HUMAN antibody
        • Apolipoprotein B (including Ag(x) antigen) antibody
        • Apolipoprotein B 100 antibody
        • Apolipoprotein B 48 antibody
        • Apolipoprotein B antibody
        • Apolipoprotein B-48 antibody
        • Apolipoprotein B100 antibody
        • Apolipoprotein B48 antibody
        • FLDB antibody
        • LDLCQ4 antibody
        see all

      Images

      • Western blot - Anti-Apolipoprotein B antibody (ab7616)
        Western blot - Anti-Apolipoprotein B antibody (ab7616)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (13)

      Publishing research using ab7616? Please let us know so that we can cite the reference in this datasheet.

      ab7616 has been referenced in 13 publications.

      • Tougan T  et al. The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. Malar J 19:155 (2020). PubMed: 32295584
      • Jia H  et al. Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. J Dairy Sci 102:833-845 (2019). PubMed: 30415861
      • Notarangelo M  et al. Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood. EBioMedicine 43:114-126 (2019). PubMed: 31047861
      • Callea F  et al. Fibrinogen Gamma Chain Mutations Provoke Fibrinogen and Apolipoprotein B Plasma Deficiency and Liver Storage. Int J Mol Sci 18:N/A (2017). PubMed: 29244742
      • Shimizu M  et al. Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPß. PLoS One 10:e0121784 (2015). WB ; Human . PubMed: 25875015
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 12 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein B antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 12 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein B antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 10 2015

      Question

      Kindly provide additional information about the conditions of WB that is shown in the image in the datasheet including the gel used.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2012

      Answer

      Thank you for contacting us.

      The gel that was used was Tris-Acetate. We use Invitrogen's NuPAGE 3-8% TA gels: http://products.invitrogen.com/ivgn/product/EA03755BOX
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Nov 08 2012

      Question

      Kindly provide additional information about the conditions of WB that is shown in the image in the datasheet.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 06 2012

      Answer

      Thank you for contacting us.
      I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
      Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentration the lane was overloaded.
      The membrane was blocked blocked for 1 hour at room temperature with 5% BSA in 0.1% TBST, with gentle rocking. Primary antibody was then diluted (1 µg/ml) with 5% BSA in 0.1% TBST and incubated overnight at 4°C with gentle rocking. The membrane was washed and incubated with Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 diluted with 5% BSA in 0.1% TBST with gentle rocking for 1 hour.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Nov 06 2012

      Question

      Do we have antiboduies suitable for immunoturbidimetry?
      ELISA or IP abs should work.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 28 2012

      Answer

      Vielen Dank für Ihren Anruf.
      Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte.
      Ich habe Ihnen daher folgende Produkte heraus gesucht:
      ab10241 Anti-Human Serum Albumin antibody [15C7]
      ab104453 Anti-Lipoprotein a antibody
      ab7616 Anti-Apolipoprotein B antibody
      Allerdings habe ich nichts für das CRP gefunden.
      Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.
      Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Aug 28 2012

      Question

      " rel="stylesheet" />
      Dear tech,

      A customer would like to askquestion about the specificity ofab7616. Whether ab7616 could react with Apo B48 and B100? Would you please help to answer? Thank you for your help!

      Best regards,
      Zhang Yingdan

      2012-05-11





      市场部
      Marketing Department
      北京市海淀区花园路B3号南楼10层 100191
      Fl.10 South Building No.3 Huayuan Road, Haidian District, Beijing, China, 100191
      电话(Tel):+86-400-8100-881
      传真(Fax):+86-10-82015198
      电子邮件(E-mail):mailto:tech@sinozhongyuan.com

      网址(Website):www.sinozhongyuan.com

      Read More

      Abcam community

      Verified customer

      Asked on May 14 2012

      Answer

      Thank you for contacting us.


      We have not mapped the epitope for ab7616 and have not tested the antibody against Apo B48 and B100. We have no information about the cross reactivity to Apo B48 and B100.

      I am sorry for not being able to provide you useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspects.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 14 2012

      Question

      Answer for your questions: In immunodiffusion test we use 0.8%agar on GelBond film.Ag and Ab are applied to holes punched in agar gels.Plasma samples from mice 5ul/hole and Ab(7616)dil.1/2(5ul).After inc(ON)the precipitin bands are visualized by staining with Comassie BrilliantBlue. Question 2:no signal Question 5 : no I don´t have the batch number of the Ab which were" successful"

      Read More

      Abcam community

      Verified customer

      Asked on Apr 18 2006

      Answer

      Thank you for getting back to us with further details. I am handling Charlottes enquiries in her absence. Once again I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been having with this antibody very much sound like a batch problem. I would be very grateful if you could provide me with some further details. If you could obtain some ordering information I would be able to trace the batches (good and bar) that you have used and I would certainly be prepared to provide you with a replacement vial; provided that the antibody was purchased within the past 90 days. I look forward to hearing from you.

      Read More

      Abcam Scientific Support

      Answered on Apr 19 2006

      Question

      I am interested in using one of your antibodies (ab7616) to bind LDL in media and prevent it from binding to the LDLR. Can you please tell me where the ab7616 binds to the apob100 lipoprotein and if it could be used in such a way?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 22 2006

      Answer

      Thank you for your enquiry. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognise a different epitope. I am not sure that the antibody can be used to prevent the Apo B100 antibody from binding to the LDL receptor, since the epitopes recognized by the antibody may not be on a portion of the protein involved in receptor binding. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Mar 22 2006

      Question

      When this antibody was tested for application in Western blotting, what type of lysate was used for characterization?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 11 2005

      Answer

      Thank you for your enquiry. When ab7616 was tested for application in Western blotting, it was tested against purified Apo B protein, which would be recommended as a positive control. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      Answered on Apr 12 2005

      Question

      I was wondering if the antibody to apolipoprotein B-100 that you sell is equal in fuction to the antibody to apolipoprotein B-100 produced by Polymedco (JI-H). Their antibody binds to the 51st residue of the protein. Do you have something like this?

      Read More

      Abcam community

      Verified customer

      Asked on May 06 2004

      Answer

      Thank you for your email. Ab7616 was made against the whole apo B and should be able to recognize all the residues. I'm not familiar with the antibody from Polymedco, so unfortunately can't state how this antibody works in comparison. If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      Answered on May 06 2004

      1-10 of 12 Abreviews or Q&A

      •  Previous
      • 1
      • 2
      • Next 

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.