Overview

  • Product name
    Anti-Apolipoprotein B antibody
    See all Apolipoprotein B primary antibodies
  • Description
    Goat polyclonal to Apolipoprotein B
  • Host species
    Goat
  • Specificity
    Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins.
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Cow, Human
  • Immunogen

    Full length native apoLipoprotein Type B (purified).

  • Positive control
    • ICC/IF: HepG2 cells.
  • General notes

    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

    At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Properties

Applications

Our Abpromise guarantee covers the use of ab7616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/5000 - 1/10000.
ELISA 1/2000 - 1/10000.
IHC-P 1/50 - 1/200.
WB 1/200 - 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in disease
    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similarities
    Contains 1 vitellogenin domain.
  • Post-translational
    modifications
    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localization
    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B (including Ag(x) antigen) antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Images

  • ab7616 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7616 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Callea F  et al. Fibrinogen Gamma Chain Mutations Provoke Fibrinogen and Apolipoprotein B Plasma Deficiency and Liver Storage. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29244742) »
  • Shimizu M  et al. Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPß. PLoS One 10:e0121784 (2015). WB ; Human . Read more (PubMed: 25875015) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Liver
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 12 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Liver
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 10 2015

Answer

Thank you for contacting us.

The gel that was used was Tris-Acetate. We use Invitrogen's NuPAGE 3-8% TA gels: http://products.invitrogen.com/ivgn/product/EA03755BOX
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.
I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentration the lane was overloaded.
The membrane was blocked blocked for 1 hour at room temperature with 5% BSA in 0.1% TBST, with gentle rocking. Primary antibody was then diluted (1 µg/ml) with 5% BSA in 0.1% TBST and incubated overnight at 4°C with gentle rocking. The membrane was washed and incubated with Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 diluted with 5% BSA in 0.1% TBST with gentle rocking for 1 hour.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Vielen Dank für Ihren Anruf.
Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte.
Ich habe Ihnen daher folgende Produkte heraus gesucht:
ab10241 Anti-Human Serum Albumin antibody [15C7]
ab104453 Anti-Lipoprotein a antibody
ab7616 Anti-Apolipoprotein B antibody
Allerdings habe ich nichts für das CRP gefunden.
Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.
Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.


We have not mapped the epitope for ab7616 and have not tested the antibody against Apo B48 and B100. We have no information about the cross reactivity to Apo B48 and B100.

I am sorry for not being able to provide you useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspects.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for getting back to us with further details. I am handling Charlottes enquiries in her absence. Once again I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been having with this antibody very much sound like a batch problem. I would be very grateful if you could provide me with some further details. If you could obtain some ordering information I would be able to trace the batches (good and bar) that you have used and I would certainly be prepared to provide you with a replacement vial; provided that the antibody was purchased within the past 90 days. I look forward to hearing from you.

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Answer

Thank you for your enquiry. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognise a different epitope. I am not sure that the antibody can be used to prevent the Apo B100 antibody from binding to the LDL receptor, since the epitopes recognized by the antibody may not be on a portion of the protein involved in receptor binding. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. When ab7616 was tested for application in Western blotting, it was tested against purified Apo B protein, which would be recommended as a positive control. If you have any additional questions, please contact us again.

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Answer

Thank you for your email. Ab7616 was made against the whole apo B and should be able to recognize all the residues. I'm not familiar with the antibody from Polymedco, so unfortunately can't state how this antibody works in comparison. If you have any more questions, please contact us again.

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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