Overview

  • Product name

    Anti-Apolipoprotein B antibody
    See all Apolipoprotein B primary antibodies
  • Description

    Goat polyclonal to Apolipoprotein B
  • Host species

    Goat
  • Specificity

    This antibody has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins.
    Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards.
    Non-specific cross-reaction of anti-apolipoprotein antibodies with other Human serum proteins is negligible.
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native Human Apolipoprotein B isolated from Human plasma.

  • Positive control

    • Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab98132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 516 kDa.
IP Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
EMSA Use at an assay dependent concentration.

Target

  • Function

    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in disease

    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similarities

    Contains 1 vitellogenin domain.
  • Post-translational
    modifications

    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apo B 100 antibody
    • Apo B antibody
    • Apo B-100 antibody
    • Apo B-48 antibody
    • Apo B100 antibody
    • Apo B48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B (including Ag(x) antigen) antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B 48 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B100 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labelled with ab98132 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab98132 staining Apolipoprotein B in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (0.5 ug/ml in BSA/TBS buffer) for 30 minutes. ab97101 Rabbit polyclonal Secondary Antibody to Anti-goat HRP, IgG - H&L, pre-adsorbed (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Chinen AB  et al. The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids. Small 13:N/A (2017). Read more (PubMed: 28196309) »
  • Podjaski C  et al. Netrin 1 regulates blood-brain barrier function and neuroinflammation. Brain 138:1598-612 (2015). WB . Read more (PubMed: 25903786) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Protein Block as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 18 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Protein Block as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 12 2013

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