Recombinant Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Apolipoprotein B antibody [EPR2914]
See all Apolipoprotein B primary antibodies -
Description
Rabbit monoclonal [EPR2914] to Apolipoprotein B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
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Positive control
- WB: Human plasma and seurm lysates; Natural Human Apolipoprotein B (ab91112)
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2914 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab139401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 515 kDa.Can be blocked with Human Apolipoprotein B peptide (ab199783). |
Target
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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Alternative names
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
Images
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![Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)](https://www.abcam.com/ps/products/139/ab139401/Images/ab139401-347173-anti-apolipoprotein-b-antibody-epr2914-westernblot-human.jpg)
Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)All lanes : Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution (Purified)
Lane 1 : Human plasma lysates at 20 µg
Lane 2 : Human serum lysates at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 515 kDa
Observed band size: 515 kDa -
![Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)](https://www.abcam.com/ps/products/139/ab139401/Images/ab139401-154821-anti-apolipoprotein-b-antibody-epr2914-western-blot.jpg)
Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)All lanes : Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution ((unpurified))
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 515 kDa
Datasheets and documents
References
ab139401 has not yet been referenced specifically in any publications.
