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RecombinantRabMAb

Recombinant Anti-Apolipoprotein B antibody [EPR2914] (ab139401)

  • Datasheet
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Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
  • Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
  • Anti-Apolipoprotein B antibody [EPR2914] (ab139401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2914] to Apolipoprotein B
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Apolipoprotein B antibody [EPR2914]
    See all Apolipoprotein B primary antibodies
  • Description

    Rabbit monoclonal [EPR2914] to Apolipoprotein B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control

    • WB: Human plasma and seurm lysates; Natural Human Apolipoprotein B (ab91112)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2914
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Fibrinolysis
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Apolipoprotein B antibody [EPR2914] - BSA and Azide free (ab231683)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Native Human Apolipoprotein B (ab91112)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein B (ab80037)

Applications

Our Abpromise guarantee covers the use of ab139401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 515 kDa.

Target

  • Function

    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in disease

    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similarities

    Contains 1 vitellogenin domain.
  • Post-translational
    modifications

    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localization

    Cytoplasm. Secreted.
  • Target information above from: UniProt accession P04114 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 338 Human
    • Omim: 107730 Human
    • SwissProt: P04114 Human
    • Unigene: 120759 Human
    • Alternative names

      • Apo B 100 antibody
      • Apo B antibody
      • Apo B-100 antibody
      • Apo B-48 antibody
      • Apo B100 antibody
      • Apo B48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B (including Ag(x) antigen) antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B 48 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B100 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    Images

    • Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
      Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
      All lanes : Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution (Purified)

      Lane 1 : Human plasma lysates at 20 µg
      Lane 2 : Human serum lysates at 20 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 515 kDa
      Observed band size: 515 kDa

    • Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
      Western blot - Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
      All lanes : Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution ((unpurified))

      Lane 1 : Human plasma lysate
      Lane 2 : Human serum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 515 kDa

    • Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
      Anti-Apolipoprotein B antibody [EPR2914] (ab139401)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab139401? Please let us know so that we can cite the reference in this datasheet.

    ab139401 has been referenced in 1 publication.

    • Sun Y  et al. Lipid Profile Characterization and Lipoprotein Comparison of Extracellular Vesicles from Human Plasma and Serum. Metabolites 9:N/A (2019). PubMed: 31683897

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab139401.
    Please use the links above to contact us or submit feedback about this product.

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