Overview

  • Product name
    Anti-Apolipoprotein CI antibody
    See all Apolipoprotein CI primary antibodies
  • Description
    Rabbit polyclonal to Apolipoprotein CI
  • Host species
    Rabbit
  • Specificity
    Ab20793 specifically binds to human Apo CI. No cross-reactions with Human Apo AI, Apo AII, Apo B-100, Apo CII, Apo CIII, or Apo E.
  • Tested applications
    Suitable for: ICC/IF, Indirect ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human Apolipoprotein-CI).

Applications

Our Abpromise guarantee covers the use of ab20793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 mg/ml.
Indirect ELISA Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 9 kDa.
ELISA 1/5000 - 1/20000. Dilution optimised using Chromogenic detection.

Target

  • Function
    Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • Tissue specificity
    Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.
  • Sequence similarities
    Belongs to the apolipoprotein C1 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APO C1 antibody
    • Apo CI antibody
    • Apo-CIB antibody
    • Apo-CIB' antibody
    • APOC 1 antibody
    • ApoC I antibody
    • ApoC-IB antibody
    • ApoC-IB' antibody
    • APOC1 antibody
    • APOC1_HUMAN antibody
    • APOC1B antibody
    • Apolipoprotein C I antibody
    • Apolipoprotein C I variant I antibody
    • Apolipoprotein C-I antibody
    • Apolipoprotein C1 antibody
    • Apolipoprotein CI antibody
    • ApolipoproteinC I antibody
    • ApolipoproteinCI antibody
    • Truncated apolipoprotein C-I antibody
    see all

Images

  • ICC/IF image of ab20793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20793, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Cheng CW  et al. Sex hormone-binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer. Cancer Med 7:64-74 (2018). WB . Read more (PubMed: 29148252) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. I now have further information to share in relation to the anti-Apolopoprotein Cl antibody (ab20793).

The antibody has been used to detect Apolopoprotein CI in an indirect ELISA. This was performed using theantigen coated on plates. The working dilution of the primary antibody for this application is suggested at 1:5000 – 1:20,000, but each lab should determine the optimal working dilution for their specific application.

A secondary antibody will need to be used in conjunction with ab20793 as this antibody is not directly conjugated itself.

I have now found the reference to the second antibody and will request this be altered to reduce the confusion.

I hope this information has been of help. If you require any further information, please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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