Overview

  • Product name

    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813]
    See all Apolipoprotein CI/Apo-CI primary antibodies
  • Description

    Rabbit monoclonal [EPR16813] to Apolipoprotein CI/Apo-CI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human Apolipoprotein CI/Apo-CI aa 1 to the C-terminus. The exact sequence is proprietary. corresponding to the mature form.
    Database link: P02654

  • Positive control

    • WB: Human fetal liver and Human plasma lysate. IHC-P: Human liver tissue. ICC/IF: HepG2 cells. FC: HeLa cells. IP: Human plasma whole cell extract .
  • General notes

     This product was previously labelled as Apolipoprotein CI

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 6.6 kDa (predicted molecular weight: 9 kDa).
IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/800.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/40.

Target

  • Function

    Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • Tissue specificity

    Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.
  • Sequence similarities

    Belongs to the apolipoprotein C1 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • APO C1 antibody
    • Apo CI antibody
    • Apo-CIB antibody
    • Apo-CIB' antibody
    • APOC 1 antibody
    • ApoC I antibody
    • ApoC-IB antibody
    • ApoC-IB' antibody
    • APOC1 antibody
    • APOC1_HUMAN antibody
    • APOC1B antibody
    • Apolipoprotein C I antibody
    • Apolipoprotein C I variant I antibody
    • Apolipoprotein C-I antibody
    • Apolipoprotein C1 antibody
    • Apolipoprotein CI antibody
    • ApolipoproteinC I antibody
    • ApolipoproteinCI antibody
    • Truncated apolipoprotein C-I antibody
    see all

Images

  • Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/1000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 9 kDa
    Observed band size: 6.6 kDa
    why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/5000 dilution + Human plasma lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 9 kDa
    Observed band size: 6.6 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/800 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198288 at 1/800 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/80 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) (ab172730) and cell without incubation with primary antibody and secondary antibody is blue.

  • Immunoprecipitation analysis of Human plasma whole cell extract labeling Apolipoprotein CI/Apo-CI using ab198288 at 1/40 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198288 in Human plasma whole cell extract.
    Lane 1: Input: 10 μg Human plasma whole cell extract.
    Subsequent WB detection was performed using ab198288 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

References

This product has been referenced in:

  • Zhang H  et al. Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis. Virology 524:1-9 (2018). Read more (PubMed: 30130702) »
  • Kim WS  et al. Altered High Density Lipoprotein Composition in Behavioral Variant Frontotemporal Dementia. Front Neurosci 12:847 (2018). Read more (PubMed: 30487733) »
See all 4 Publications for this product

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