Anti-Apolipoprotein CII/ApoC-II antibody (ab230447)

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Overview

  • Product name

    Anti-Apolipoprotein CII/ApoC-II antibody
    See all Apolipoprotein CII/ApoC-II primary antibodies

  • Description

    Rabbit polyclonal to Apolipoprotein CII/ApoC-II

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Human Apolipoprotein CII/ApoC-II.
    Database link: P02655

  • Positive control

    • IHC-P: Human placenta tissue. WB: HL-60 and A549 cell extracts.

  • General notes

     This product was previously labelled as Apolipoprotein CII

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 11 kDa.

Target

  • Function

    Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.

  • Tissue specificity

    Liver and intestine.

  • Involvement in disease

    Hyperlipoproteinemia 1B

  • Sequence similarities

    Belongs to the apolipoprotein C2 family.

  • Post-translational
    modifications

    Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing.
    Proapolipoprotein C-II, the major form found in plasma undergoes proteolytic cleavage of its N-terminal hexapeptide to generate apolipoprotein C-II, which occurs as the minor form in plasma.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • APC 2 antibody
    • APC2 antibody
    • Apo CII antibody
    • Apo-CII antibody
    • APOC 2 antibody
    • ApoC II antibody
    • ApoC-II antibody
    • APOC2 antibody
    • APOC2 protein antibody
    • APOC2_HUMAN antibody
    • ApoCII antibody
    • Apolipoprotein C II antibody
    • Apolipoprotein C II precursor antibody
    • Apolipoprotein C2 antibody
    • ApolipoproteinCII antibody
    • MGC75082 antibody
    • ProapoC-II antibody
    • Proapolipoprotein C-II antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CII/ApoC-II antibody (ab230447)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CII/ApoC-II antibody (ab230447)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Apolipoprotein CII/ApoC-II using ab230447 at 1/200 dilution in immunohistochemical analysis.

  • Western blot - Anti-Apolipoprotein CII/ApoC-II antibody (ab230447)
    Western blot - Anti-Apolipoprotein CII/ApoC-II antibody (ab230447)
    All lanes : Anti-Apolipoprotein CII/ApoC-II antibody (ab230447) at 1/500 dilution

    Lane 1 : HL-60 (Human promyelocytic leukemia cell line) cell extract
    Lane 2 : A549 (human lung carcinoma cell line) cell extract

    Predicted band size: 11 kDa

References

ab230447 has not yet been referenced specifically in any publications.

Publishing research using ab230447? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-Apolipoprotein CII antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Serum (murine serum)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
2 µg
Treatment
tyloxapol, oil gavage
Specification
murine serum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Aug 01 2018

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