Overview

  • Product name

    Anti-Apolipoprotein CII/ApoC-II antibody
    See all Apolipoprotein CII/ApoC-II primary antibodies
  • Description

    Rabbit polyclonal to Apolipoprotein CII/ApoC-II
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Apolipoprotein CII/ApoC-II aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88220)

  • Positive control

    • This antibody gave a positive signal in Human Plasma Total Protein Lysate. IHC-P: human normal liver FFPE tissue sections
  • General notes

     This product was previously labelled as Apolipoprotein CII

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
  • Tissue specificity

    Liver and intestine.
  • Involvement in disease

    Hyperlipoproteinemia 1B
  • Sequence similarities

    Belongs to the apolipoprotein C2 family.
  • Post-translational
    modifications

    Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing.
    Proapolipoprotein C-II, the major form found in plasma undergoes proteolytic cleavage of its N-terminal hexapeptide to generate apolipoprotein C-II, which occurs as the minor form in plasma.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC 2 antibody
    • APC2 antibody
    • Apo CII antibody
    • Apo-CII antibody
    • APOC 2 antibody
    • ApoC II antibody
    • ApoC-II antibody
    • APOC2 antibody
    • APOC2 protein antibody
    • APOC2_HUMAN antibody
    • ApoCII antibody
    • Apolipoprotein C II antibody
    • Apolipoprotein C II precursor antibody
    • Apolipoprotein C2 antibody
    • ApolipoproteinCII antibody
    • MGC75082 antibody
    • ProapoC-II antibody
    • Proapolipoprotein C-II antibody
    see all

Images

  • Anti-Apolipoprotein CII/ApoC-II antibody (ab76452) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 9 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    The Apolipoprotein CII/ApoC-II protein has a predicted molecular weight of 11-kDa. The first 22 amino-acids of the Apolipoprotein CII/ApoC-II sequence act as a signal sequence, and when cleaved the protein has an expected molecular weight of 9-kDa.

  • ICC/IF image of ab76452 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76452, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml
  • IHC image of Apolipoprotein CII/ApoC-II staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76452, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Okubo M  et al. Apolipoprotein C-II Tuzla: a novel large deletion in APOC2 caused by Alu-Alu homologous recombination in an infant with apolipoprotein C-II deficiency. Clin Chim Acta 438:148-53 (2015). WB ; Human . Read more (PubMed: 25172036) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Isoelectrofocusing pH4-6.5)
Sample
Human Serum (d<1.006 fraction isolated by ultracentrifuge)
Specification
d<1.006 fraction isolated by ultracentrifuge
Treatment
dissolved in 8M Urea
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

実 大久保

Verified customer

Submitted Sep 17 2014

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