Western blot abreview for Anti-Apolipoprotein CII antibody

Good
Abreviews
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Isoelectrofocusing pH4-6.5)
Sample
Human Serum (d<1.006 fraction isolated by ultracentrifuge)
Specification
d<1.006 fraction isolated by ultracentrifuge
Treatment
dissolved in 8M Urea
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000

Detection

Detection method
ECL prime
Bands
Specific: 8.8 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
d<1.006 fraction from a patient with apo C-II deficiency (apo C-II Tuzla)
Positive control
apo C-II protein from human plasma purchased from Sigma-Aldrich (St. Louis, USA)

Additional data

Additional Notes
Isoelectrofocusing (IEF) pH 4-6.5 of apo C-II and western blotting Okubo M, et al. Apolipoprotein C-II Tuzla: A novel large deletion in APOC2 caused by Alu-Alu homologous recombination in an infant with apolipoprotein C-II deficiency. Clin Chim Acta. 2014 Aug 27438C:148-153. doi: 10.1016/j.cca.2014.08.022. [Epub ahead of print]
PubMed ID
25172036: View

実 大久保

Verified customer

Submitted Sep 17 2014

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