Product nameAnti-Apolipoprotein CIII antibody
See all Apolipoprotein CIII primary antibodies
DescriptionRabbit polyclonal to Apolipoprotein CIII
SpecificitySpecifically binds to human Apo CIII.
Tested applicationsSuitable for: Sandwich ELISA, ICC/IF, ELISA, WBmore details
Species reactivityReacts with: Human, Monkey
Full length protein corresponding to Human Apolipoprotein CIII .
- ELISA: Human or cynomolgus monkey serum. WB: Natural Human Apolipoprotein CIII. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.0292% EDTA, 0.435% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by Human Apo CIII-Sepharose affinity column.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
ELISA pair antibody
Our Abpromise guarantee covers the use of ab21032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. PubMed: 21371998|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ELISA||1/5000 - 1/40000. Can be paired for ELISA with Goat polyclonal to Apolipoprotein CIII (Biotin) (ab21024).|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.|
FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Sequence similaritiesBelongs to the apolipoprotein C3 family.
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
- Information by UniProt
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
Sandwich ELISA, detecting Apolipoprotein CIII levels in human or cynomolgus monkey serum, using ab21032 as capture antibody.
Anti-human Apolipoprotein CIII polyclonal antibody (ab21032 at 10 µg/ml) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.
All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml
Lane 1 :
Natural Human Apolipoprotein CIII (ab77944) at 0.1 µg
Lane 2 :
Natural Human Apolipoprotein CIII (ab77944) at 0.01 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute
ICC/IF image of ab21032 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells.
The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa-Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
This product has been referenced in:
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). Read more (PubMed: 28825717) »
- Schjoldager KT et al. Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells. Proc Natl Acad Sci U S A 109:9893-8 (2012). IP ; Human . Read more (PubMed: 22566642) »