• Product name

    Anti-Apolipoprotein CIII antibody
    See all Apolipoprotein CIII primary antibodies
  • Description

    Goat polyclonal to Apolipoprotein CIII
  • Host species

  • Specificity

    Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins.
  • Tested applications

    Suitable for: ELISA, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human, Syrian hamster
  • Immunogen

    Full length native apoLipoprotein Type C-III (purified).

  • General notes

    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.



Our Abpromise guarantee covers the use of ab7619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/10000.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/5000.
IP 1/100.


  • Function

    Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificity

    Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in disease

    Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • Sequence similarities

    Belongs to the apolipoprotein C3 family.
  • Post-translational

    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all


  • IHC image of ab7619 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). IHC-P ; Mouse . Read more (PubMed: 24186947) »
  • Qu S  et al. PPAR{alpha} mediates the hypolipidemic action of fibrates by antagonizing FoxO1. Am J Physiol Endocrinol Metab 292:E421-34 (2007). WB ; Syrian hamster . Read more (PubMed: 16985262) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you again for your enquiry and I apologize for the delay in responding to you. There is no Western blot image available from us for this antibody. This antibody has been used and the Western images published in the following reference, however, Altomonte J et al. Foxo1 mediates insulin action on apoC-III and triglyceride metabolism. J Clin Invest 114:1493-503 (2004). PubMed: 15546000. In this reference the antibody is used on 20 ug of plasma proteins at 1/2000 and the secondary is used at 1/1000. I noticed that you have tried the secondary at 1/40,000. I am wondering if you could try the primary at 1/1000 without milk and the secondary at 1/1000 without milk. Perhaps by concentrating the secondary and removing the milk you will see the correct band. You may also benefit from contacting the corresponding author of the paper Hengjian Henry Dong, Email: dongh@pitt.edu. Please let me know if this helps and do not hesitate to contact us for further advice,

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We apply purified apolipoprotein C-III to the gel and get only a single band at 8.8 kDa. A picture is not available. tThe suggested dilution is 1/5000 to 1/10,000. Human serum would be a good western sample. I hope this information helps you. If you have any further questions please do not hesitate in contacting me.

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