Key features and details
- Goat polyclonal to Apolipoprotein CIII (Biotin)
- Suitable for: Sandwich ELISA, WB, ELISA
- Reacts with: Human, Monkey
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Apolipoprotein CIII antibody (Biotin)
See all Apolipoprotein CIII primary antibodies
DescriptionGoat polyclonal to Apolipoprotein CIII (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
Species reactivityReacts with: Human, Monkey
Purified human Apolipoprotein CIII.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.0268% PBS, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesHuman Apo CIII-Sepharose affinity column and labeled with Biotin.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
ELISA pair antibody
Our Abpromise guarantee covers the use of ab21024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. PubMed: 21371998|
|WB||1/8000 - 1/30000. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.|
|ELISA||1/8000 - 1/30000. Can be paired for ELISA with Rabbit polyclonal to Apolipoprotein CIII (ab21032).|
FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Sequence similaritiesBelongs to the apolipoprotein C3 family.
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
- Information by UniProt
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21024 as detection antibody.
Anti-human Apolipoprotein CIII polyclonal antibody (ab21032) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024 at 1 µg/ml) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab21024 has been referenced in 3 publications.
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus monkey . PubMed: 21371998
- Dernick G et al. Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays. J Lipid Res 52:2323-31 (2011). PubMed: 21971713