Product nameAnti-Apolipoprotein CIII antibody (Biotin)
See all Apolipoprotein CIII primary antibodies
DescriptionGoat polyclonal to Apolipoprotein CIII (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
Species reactivityReacts with: Human, Monkey
Purified human Apolipoprotein CIII.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.0268% PBS, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesHuman Apo CIII-Sepharose affinity column and labeled with Biotin.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
ELISA pair antibody
Our Abpromise guarantee covers the use of ab21024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. PubMed: 21371998|
|WB||1/8000 - 1/30000. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.|
|ELISA||1/8000 - 1/30000. Can be paired for ELISA with Rabbit polyclonal to Apolipoprotein CIII (ab21032).|
FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Sequence similaritiesBelongs to the apolipoprotein C3 family.
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
- Information by UniProt
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21024 as detection antibody.
Anti-human Apolipoprotein CIII polyclonal antibody (ab21032) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024 at 1 µg/ml) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.
This product has been referenced in:
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). Read more (PubMed: 28825717) »
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus monkey . Read more (PubMed: 21371998) »