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RabMAb

Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)

  • Datasheet
Reviews (4)Q&A (1)References (4)

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Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)

    Key features and details

    • Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
    • Suitable for: WB, IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Apolipoprotein CIII (ab157899)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Apolipoprotein CIII antibody [EP1372Y]
      See all Apolipoprotein CIII primary antibodies
    • Description

      Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WB, IPmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human Apolipoprotein CIII aa 50-150 (C terminal). The exact sequence is proprietary.

    • Positive control

      • Human liver tissue lysate, Rat primary astrocytes.
    • General notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.20
      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      EP1372Y
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Recombinant Protein

      • Recombinant Human Apolipoprotein CIII (ab157899)

    Applications

    Our Abpromise guarantee covers the use of ab76305 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
    IP 1/30.

    Target

    • Function

      Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
    • Tissue specificity

      Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
    • Involvement in disease

      Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    • Sequence similarities

      Belongs to the apolipoprotein C3 family.
    • Post-translational
      modifications

      O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02656 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 345 Human
      • Omim: 107720 Human
      • SwissProt: P02656 Human
      • Unigene: 73849 Human
      • Alternative names

        • APOC3 antibody
        • APO C3 antibody
        • Apo CIII antibody
        • Apo-CIII antibody
        • APOC 3 antibody
        • ApoC III antibody
        • ApoC-III antibody
        • APOC3 antibody
        • APOC3_HUMAN antibody
        • ApoCIII antibody
        • Apolipoprotein C III antibody
        • Apolipoprotein C-III antibody
        • Apolipoprotein C3 antibody
        • ApolipoproteinCIII antibody
        • MGC150353 antibody
        see all

      Images

      • Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)
        Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)
        Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 11 kDa
        Observed band size: 11 kDa

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab76305? Please let us know so that we can cite the reference in this datasheet.

      ab76305 has been referenced in 4 publications.

      • Li L  et al. Proteomics analysis of potential serum biomarkers for insulin resistance in patients with polycystic ovary syndrome. Int J Mol Med 45:1409-1416 (2020). PubMed: 32323743
      • Lv P  et al. Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function. Int J Mol Med 41:61-68 (2018). PubMed: 29115584
      • Zhang H  et al. Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis. Virology 524:1-9 (2018). PubMed: 30130702
      • Wang Y  et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). PubMed: 21371998

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Apolipoprotein CIII antibody [EP1372Y]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Primary astrocytes culture)
      Permeabilization
      Yes - 0.1M PBS with 1% Triton X
      Specification
      Primary astrocytes culture
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
      Fixative
      Methanol
      Read More

      Dr. Ruma Raha-Chowdhury

      Verified customer

      Submitted Sep 06 2016

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Apolipoprotein CIII antibody [EP1372Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Liver)
      Permeabilization
      Yes - 0.1 M PBS with 3% Triton X
      Specification
      Liver
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Ruma Raha-Chowdhury

      Verified customer

      Submitted Feb 05 2016

      Question

      Customer kindly asked the blocking buffer used in WB, antibody diluent and the reason why ab19345 is detecting band at 90kDa instead of 325kDa..

      Read More

      Abcam community

      Verified customer

      Asked on Mar 13 2012

      Answer

      Thank you for contacting us.

      Please find attached the WB protocol;

      https://www.abcam.com/index.html?pageconfig=resource&rid=13045

      Please find attached the link which actually explains the reason of band at ˜70-80 kDa. This is the cleavage product of versican. Please refer to fig 1.

      Please be also advised that there are 5 isoforms of versican protein i.e. V0, V1, V2, V3, Vint. http://www.uniprot.org/uniprot/P13611
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Mar 14 2012

      Western blot abreview for Anti-Apolipoprotein CIII antibody [EP1372Y]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (BREAST (MCF-7))
      Loading amount
      0.2 µg
      Specification
      BREAST (MCF-7)
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 01 2010

      Flow Cytometry abreview for Anti-Apolipoprotein CIII antibody [EP1372Y]

      Inconclusive
      Abreviews
      Abreviews
      Application
      Flow Cytometry
      Sample
      Human Cell (U266 cell line B lymphocyte (myeloma))
      Permeabilization
      No
      Gating Strategy
      total stained cells
      Specification
      U266 cell line B lymphocyte (myeloma)
      Preparation
      Cell harvesting/tissue preparation method: suspension cells, so used directly
      Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
      Fixation
      Unfixed
      Read More

      Prof. Paul Townsend

      Verified customer

      Submitted May 07 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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