Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)
![Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)](https://www.abcam.com/ps/products/76/ab76305/Images/ab76305-91686-anti-apolipoprotein-ciii-antibody-ep1372y-western-blot.jpg "Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)")
Key features and details
- Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apolipoprotein CIII antibody [EP1372Y]
See all Apolipoprotein CIII primary antibodies -
Description
Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Apolipoprotein CIII aa 50-150 (C terminal). The exact sequence is proprietary.
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Positive control
- Human liver tissue lysate, Rat primary astrocytes.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1372Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). | |
| IP | 1/30. |
Target
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Function
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
Tissue specificity
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
Involvement in disease
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
Sequence similarities
Belongs to the apolipoprotein C3 family. -
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
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Alternative names
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
Images
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Western blot - Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Protocols
Datasheets and documents
References (4)
ab76305 has been referenced in 4 publications.
- Li L et al. Proteomics analysis of potential serum biomarkers for insulin resistance in patients with polycystic ovary syndrome. Int J Mol Med 45:1409-1416 (2020). PubMed: 32323743
- Lv P et al. Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function. Int J Mol Med 41:61-68 (2018). PubMed: 29115584
- Zhang H et al. Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis. Virology 524:1-9 (2018). PubMed: 30130702
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). PubMed: 21371998
