Recombinant
RabMAb

Recombinant Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)

Overview

  • Product name

    Anti-Apolipoprotein CIII antibody [EPR1371(2)]
    See all Apolipoprotein CIII primary antibodies
  • Description

    Rabbit monoclonal [EPR1371(2)] to Apolipoprotein CIII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Apolipoprotein CIII aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: lysate from Human plasma or HDL cells; IHC-P: Human liver tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
    • Tissue specificity

      Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
    • Involvement in disease

      Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    • Sequence similarities

      Belongs to the apolipoprotein C3 family.
    • Post-translational
      modifications

      O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • APOC3 antibody
      • APO C3 antibody
      • Apo CIII antibody
      • Apo-CIII antibody
      • APOC 3 antibody
      • ApoC III antibody
      • ApoC-III antibody
      • APOC3 antibody
      • APOC3_HUMAN antibody
      • ApoCIII antibody
      • Apolipoprotein C III antibody
      • Apolipoprotein C-III antibody
      • Apolipoprotein C3 antibody
      • ApolipoproteinCIII antibody
      • MGC150353 antibody
      see all

    Images

    • All lanes : Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205) at 1/1000 dilution

      Lane 1 : Human plasma
      Lane 2 : HDL cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa

    • ab108205 at 1/250 dilution staining Apolipoprotein CIII in paraffin-embedded Human liver tissue by immunohistochemistry,.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab108205 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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