Recombinant
RabMAb

Anti-Apolipoprotein L 1 antibody [EPR2907(2)] - BSA and Azide free (ab169952)

Overview

  • Product name
    Anti-Apolipoprotein L 1 antibody [EPR2907(2)] - BSA and Azide free
    See all Apolipoprotein L 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L 1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IP, ICC, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Apolipoprotein L 1. The exact sequence is proprietary.

  • General notes

    ab169952 is a PBS-only buffer formulated version of ab108315, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab108315 for information on protocos, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.

Target

  • Function
    May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
  • Tissue specificity
    Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
  • Involvement in disease
    Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities
    Belongs to the apolipoprotein L family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APO L antibody
    • Apo-L antibody
    • ApoL antibody
    • APOL I antibody
    • ApoL-I antibody
    • APOL1 antibody
    • APOL1_HUMAN antibody
    • APOLI antibody
    • Apolipoprotein L antibody
    • Apolipoprotein L I antibody
    • Apolipoprotein L-I antibody
    • Apolipoprotein L1 antibody
    • FSGS4 antibody
    see all

Images

  • This IP data was generated using the same anti-Apolipoprotein L1 antibody clone, EPR2907(2), in a different buffer formulation (cat# ab108315).

    Apolipoprotein L 1  was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

    Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

    Lane 1:Human plasma

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • This Flow Cyt data was generated using the same anti-Apolipoprotein L1 antibody clone, EPR2907(2), in a different buffer formulation (cat# ab108315).

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab169952 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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