Anti-Apolipoprotein L1/APOL1 antibody (ab231523)
Key features and details
- Rabbit polyclonal to Apolipoprotein L1/APOL1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apolipoprotein L1/APOL1 antibody
See all Apolipoprotein L1/APOL1 primary antibodies -
Description
Rabbit polyclonal to Apolipoprotein L1/APOL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Apolipoprotein L1/APOL1 aa 1-250. N-terminal His-tag and GST-tag. Expressed in E.coli.
Database link: O14791 -
Positive control
- IHC-P: Human kidney tissue. WB: MCF7 cell lysate; Recombinant human Apolipoprotein L 1/APOL1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231523 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa. |
Target
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Function
May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. -
Tissue specificity
Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen. -
Involvement in disease
Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Belongs to the apolipoprotein L family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8542 Human
- Omim: 603743 Human
- SwissProt: O14791 Human
- Unigene: 114309 Human
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Alternative names
- APO L antibody
- Apo-L antibody
- ApoL antibody
see all
Images
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Anti-Apolipoprotein L1/APOL1 antibody (ab231523) at 3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate
Secondary
HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 44 kDa -
Paraffin-embedded human kidney tissue stained for Apolipoprotein L 1/APOL1 using ab231523 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Apolipoprotein L1/APOL1 antibody (ab231523) at 5 µg/ml + Recombinant human Apolipoprotein L 1/APOL1 protein
Predicted band size: 44 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231523 has been referenced in 1 publication.
- Zhang Z et al. Recipient APOL1 risk alleles associate with death-censored renal allograft survival and rejection episodes. J Clin Invest 131:N/A (2021). PubMed: 34499625