Key features and details
- Rabbit polyclonal to Apolipoprotein L1/APOL1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Apolipoprotein L1/APOL1 antibody
See all Apolipoprotein L1/APOL1 primary antibodies
DescriptionRabbit polyclonal to Apolipoprotein L1/APOL1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Apolipoprotein L1/APOL1 aa 1-238. N-terminal His-tag and GST-tag. Expressed in E.coli.
MEGAALLRVSVLCIWMSALFLGVGVRAEEAGARVQQNVPSGTDTGDPQSK PLGDWAAGTMDPESSIFIEDAIKYFKEKVSTQNLLLLLTDNEAWNGFVAA AELPRNEADELRKALDNLARQMIMKDKNWHDKGQQYRNWFLKEFPRLKSE LEDNIRRLRALADGVQKVHKGTTIANVVSGSLSISSGILTLVGMGLAPFT EGGSLVLLEPGMELGITAALTGITSSTMDYGKKWWTQA
Database link: O14791
- IHC-P: Human kidney tissue. WB: MCF7 cell lysate; Recombinant human Apolipoprotein L 1/APOL1 protein.
This product was previously labelled as Apolipoprotein L 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231523 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.|
FunctionMay play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Tissue specificityPlasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
Involvement in diseaseDefects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesBelongs to the apolipoprotein L family.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- APO L antibody
- Apo-L antibody
- ApoL antibody
Anti-Apolipoprotein L1/APOL1 antibody (ab231523) at 3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate
HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Paraffin-embedded human kidney tissue stained for Apolipoprotein L 1/APOL1 using ab231523 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Apolipoprotein L1/APOL1 antibody (ab231523) at 5 µg/ml + Recombinant human Apolipoprotein L 1/APOL1 protein
Predicted band size: 44 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231523 has not yet been referenced specifically in any publications.