Key features and details
- Goat polyclonal to Apolipoprotein L1/APOL1
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Apolipoprotein L1/APOL1 antibody
See all Apolipoprotein L1/APOL1 primary antibodies
DescriptionGoat polyclonal to Apolipoprotein L1/APOL1
Specificityab79282 is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1).
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
- Human Brain (Frontal Cortex) lysate.
This product was previously labelled as Apolipoprotein L 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab79282 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Our Abpromise guarantee covers the use of ab79282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
1 hour primary incubation is recommended for this product.
FunctionMay play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Tissue specificityPlasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
Involvement in diseaseDefects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesBelongs to the apolipoprotein L family.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- APO L antibody
- Apo-L antibody
- ApoL antibody
Anti-Apolipoprotein L1/APOL1 antibody (ab79282) at 1 µg/ml + Human Frontal Cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 44 kDa
Observed band size: 44 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab79282 (0.1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ab79282 has not yet been referenced specifically in any publications.