Recombinant
RabMAb

Recombinant Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

Overview

  • Product name

    Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)]
    See all Apolipoprotein L1/APOL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Apolipoprotein L1/APOL1 aa 300-400.
    (Peptide available as ab194579)

  • Positive control

    • Human plasma, ovary cancer, and HepG2 cell lysates.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Apolipoprotein L 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
    • Tissue specificity

      Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
    • Involvement in disease

      Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
    • Sequence similarities

      Belongs to the apolipoprotein L family.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • APO L antibody
      • Apo-L antibody
      • ApoL antibody
      • APOL I antibody
      • ApoL-I antibody
      • APOL1 antibody
      • APOL1_HUMAN antibody
      • APOLI antibody
      • Apolipoprotein L antibody
      • Apolipoprotein L I antibody
      • Apolipoprotein L-I antibody
      • Apolipoprotein L1 antibody
      • FSGS4 antibody
      see all

    Images

    • All lanes : Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    • Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

      Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

      Lane 1:Human plasma

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    References

    This product has been referenced in:

    See all 9 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RCC 786-0)
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage Bis-Tris)
    Loading amount
    10 µg
    Specification
    RCC 786-0
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Aug 22 2017

    Question
    Answer

    The lab uses Gibco FreeStyle 293 Expression medium, which is serum and protein free.

    Read More

    Answer

    For ab126615, I have attached the WB image with HepG2 cell lysate.

    As for the cell lysis buffer used for the WB with ab108315, it is product ab152163 (https://www.abcam.com/index.html?datasheet=152163) that was used. The buffer composition is given on the datasheet:
    pH: 7.50
    Constituents: 0.216% Beta glycerophosphate, 0.19% Sodium orthovanadate, 0.001% Leupeptin, 0.38% EGTA, 10% Triton-X-100, 3.15% Tris HCl, 8.8% Sodium chloride, 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate

    Read More

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