Question (72147) | Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

Go to datasheet (ab108315)

Question

ab126615: Why no HepG2 cell lysate used for WB? (For ab108315, HepG2 cells were used?)
ab108315: What was used as cell lysis buffer?

Answer

For ab126615, I have attached the WB image with HepG2 cell lysate.

As for the cell lysis buffer used for the WB with ab108315, it is product ab152163 (https://www.abcam.com/index.html?datasheet=152163) that was used. The buffer composition is given on the datasheet:
pH: 7.50
Constituents: 0.216% Beta glycerophosphate, 0.19% Sodium orthovanadate, 0.001% Leupeptin, 0.38% EGTA, 10% Triton-X-100, 3.15% Tris HCl, 8.8% Sodium chloride, 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate