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ab126615: Why no HepG2 cell lysate used for WB? (For ab108315, HepG2 cells were used?)
ab108315: What was used as cell lysis buffer?
Asked on Feb 19 2013
For ab126615, I have attached the WB image with HepG2 cell lysate.
As for the cell lysis buffer used for the WB with ab108315, it is product ab152163 (https://www.abcam.com/index.html?datasheet=152163) that was used. The buffer composition is given on the datasheet:
Constituents: 0.216% Beta glycerophosphate, 0.19% Sodium orthovanadate, 0.001% Leupeptin, 0.38% EGTA, 10% Triton-X-100, 3.15% Tris HCl, 8.8% Sodium chloride, 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate
Answered on Feb 19 2013