Recombinant
RabMAb

Recombinant Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] - BSA and Azide free (ab169952)

Overview

  • Product name

    Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] - BSA and Azide free
    See all Apolipoprotein L1/APOL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Apolipoprotein L1/APOL1. The exact sequence is proprietary.

  • General notes

    ab169952 is the carrier-free version of ab108315 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab169952 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Apolipoprotein L 1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.

Target

  • Function

    May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
  • Tissue specificity

    Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
  • Involvement in disease

    Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities

    Belongs to the apolipoprotein L family.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • APO L antibody
    • Apo-L antibody
    • ApoL antibody
    • APOL I antibody
    • ApoL-I antibody
    • APOL1 antibody
    • APOL1_HUMAN antibody
    • APOLI antibody
    • Apolipoprotein L antibody
    • Apolipoprotein L I antibody
    • Apolipoprotein L-I antibody
    • Apolipoprotein L1 antibody
    • FSGS4 antibody
    see all

Images

  • This IP data was generated using the same anti-Apolipoprotein L 1/APOL1 antibody clone, EPR2907(2), in a different buffer formulation (cat# ab108315).

    Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

    Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

    Lane 1:Human plasma

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • This Flow Cyt data was generated using the same anti-Apolipoprotein L 1/APOL1 antibody clone, EPR2907(2), in a different buffer formulation (cat# ab108315).

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab169952 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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