Product nameAnti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] - BSA and Azide free
See all Apolipoprotein L1/APOL1 primary antibodies
DescriptionRabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, IP, ICC, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Apolipoprotein L1/APOL1. The exact sequence is proprietary.
ab169952 is the carrier-free version of ab108315 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab169952 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Apolipoprotein L 1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.|
FunctionMay play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Tissue specificityPlasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen.
Involvement in diseaseDefects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesBelongs to the apolipoprotein L family.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- APO L antibody
- Apo-L antibody
- ApoL antibody
This IP data was generated using the same anti-Apolipoprotein L 1/APOL1 antibody clone, EPR2907(2), in a different buffer formulation (cat# ab108315).
Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.
Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.
Lane 1:Human plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This Flow Cyt data was generated using the same anti-Apolipoprotein L 1/APOL1 antibody clone, EPR2907(2), in a different buffer formulation (cat# ab108315).
Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
ab169952 has not yet been referenced specifically in any publications.