Overview

  • Product name

    Anti-Apolipoprotein O like antibody
  • Description

    Rabbit polyclonal to Apolipoprotein O like
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human Apolipoprotein O like aa 145-260.
    Sequence:

    ATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPK PKEKTKLGSSSEIEVPAKTTHVLKHSVPLPTELSSEAKTKSESTSGATQF MPDPKLMDHGQSHPED


    Database link: Q6UXV4

  • Positive control

    • IHC: Human stomach tissue. WB: HeLa, NIH/3T3 and NBT-II cell lysate. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB 1/250 - 1/500. Predicted molecular weight: 29 kDa.

Target

Images

  • Anti-Apolipoprotein O like antibody (ab224036) at 1/250 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 29 kDa

  • Paraffin-embedded human stomach tissue stained for Apolipoprotein O like using ab224036 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Apolipoprotein O like antibody (ab224036) at 1/250 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : NBT-II (rat cell line) whole cell lysate

    Predicted band size: 29 kDa

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Apolipoprotein O like (green) using ab224036 at 4 µg/ml in ICC/IF.

References

ab224036 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224036.
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