Overview

  • Product name

    Apoptosis DNA Ladder Assay Kit
    See all DNA fragmentation kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Direct
  • Assay time

    1h 30m
  • Product overview

    Apoptosis DNA Ladder Assay Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes. No DNA extraction or additional columns required.


    Apoptosis DNA ladder assay protocol summary:
    - pellet cells by spinning
    - wash cells with PBS and spinning
    - lyse cells with TE lysis buffer and pipetting
    - add enzyme A solution and incubate for 10 min
    - add enzyme B solution and incubate for 30 min
    - add ammonium acetate solution, isopropanol and freeze for 10 min
    - spin to preciptate DNA
    - wash pellet with 70% ethanol, air-dry and dissolve in DNA suspension buffer
    - run on agarose gel and visualize with DNA staining dye

  • Notes

    This product was previously called Apoptotic DNA Ladder Detection Kit. 

    For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.

Properties

Images

  • Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.

Protocols

References

This product has been referenced in:

  • Li X  et al. The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network. Oxid Med Cell Longev 2019:9706792 (2019). Read more (PubMed: 30911355) »
  • Park E  et al. Ethanol Extract of Evodia rutaecarpa Attenuates Cell Growth through Caspase-Dependent Apoptosis in Benign Prostatic Hyperplasia-1 Cells. Nutrients 10:N/A (2018). Read more (PubMed: 29690562) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

If you choose to do an overnight incubation with Enzyme B, we recommend doing so at 50 degrees Celsius. If you do this, make sure the tube is sealed well to prevent evaporation.



As long as you perform the incubation for at least 30 minutes, you should obtain good results. Doing the incubation overnight does not necessarily give better results; it is offered more as an option for flexibility if researchers want to perform the incubation overnight.



If you want to create a postive control apoptosis induced sample, we carry a variety of apoptosis inducers to add to your cultured cells. Please see them through the link below:



https://www.abcam.com/products?keywords=apoptosis+inducer&selected.classification=Agonists%2C+activators%2C+antagonists+and+inhibitors

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It is very easy to use.

Excellent Excellent 5/5 (Ease of Use)
Abreviews
very good for menuscript data

Miss. Jae Ok Seo

Verified customer

Submitted Jul 23 2014

Answer

While it is uncommon for the DNA pellet to not break up with vortexing and pippetting, should this occur warming the solutions should help. There is also a yellow dye included in the suspension buffer for visualizing on the gel.

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Answer

The lab has confirmed that you may store the resuspended DNA at -20°C. DNA generally does not freeze at this temp. Please don’t store it at 4°C.

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Answer

Thank you for your enquiry.

For India, you can order from one of our following official distributors. I can recommend to contact them and they can give you a price.

Central and South India:

Allied Scientific Products
39/19B Gopal Nagar Road
Kolkata-700027
India


Website http://www.alliedscientific.net
Freephone 1800 345 7777
Fax +91 33 24566683 / 24790626
Tel +91 33 24566684/24793109/9039

North India:


NuLife Consultants and Distributors (Pvt.) Ltd.

NuLife Consultants and Distributors (Pvt.) Ltd.
M-52, First Floor,
Lajpat Nagar- Part-II,
New Delhi-110024-India
Tel 011-46112691/ 46112692/ 29836141
Website http://www.nulife.in/
Fax 91 - 011-46586216
Email mailto:nulife@bol.net.in
mailto:sales@nulife.in

I hope this will be helpful to you. if you have any further questions, please do not hesitate to contact me.

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Answer

Thank you for contacting Abcam.

I have had the chance to speak with the lab. Although they have not seen this type of issue before, they suggest when doing overnight incubation to ensure that the tubes are airtight to prevent evaporation of the samples.



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for your enquiry. I am pleased to confirm the following information for you regarding the components of this kit (ab66090) TE Lysis Buffer- Aids in cell lysis Enzyme A Solution- Removes RNA Enzyme B (Lyophilized)- Digests proteins (proteinase) Ammonium Acetate Solution- Helps in DNA precipitation DNA Suspension Buffer- Used to resuspend purified DNA I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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