Product nameApoptosis/ Necrosis Assay Kit (blue, green, red)
See all Apoptosis/ Necrosis kits
Sample typeAdherent cells, Suspension cells
Assay time1h 00m
Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.
The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.
Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.
Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.
In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm.
This kit is optimized to simultaneously detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.
This product was previously called Apoptosis/ Necrosis Detection Kit.
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
PlatformFlow cytometer, Fluorescence microscope
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests 200X 7-AAD 1 x 100µl Apopxin Green Indicator 1 x 200µl Assay Buffer 1 x 50ml CytoCalcein Violet 450 1 vial
Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.
Fluorescent analysis of live non-induced Jurkat cells stained by CytoCalcein Violet 450.
Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37 oC, 5% CO2 incubator for 5 hours, and then
loaded with Apopxin Green Indicator for 30 minutes. The fluorescence intensity of Apopxin Green Indicator was measured with a
flow cytometer using FL1 channel.
This product has been referenced in:
- Fichtman B et al. Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy. Nat Commun 10:605 (2019). Read more (PubMed: 30723199) »
- Ou K et al. Müller Cells Stabilize Microvasculature through Hypoxic Preconditioning. Cell Physiol Biochem 52:668-680 (2019). Read more (PubMed: 30921506) »