Key features and details
- Assay type: Cell-based
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
Product nameApoptosis/ Necrosis Assay Kit (blue, green, red)
See all Apoptosis/ Necrosis kits
Sample typeAdherent cells, Suspension cells
Assay time1h 00m
Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.
The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.
Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.
Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.
In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm.
This kit is optimized to simultaneously detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.
This product was previously called Apoptosis/ Necrosis Detection Kit.
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
PlatformFlow cytometer, Fluorescence microscope
Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.
Fluorescent analysis of live non-induced Jurkat cells stained by CytoCalcein Violet 450.
Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37 oC, 5% CO2 incubator for 5 hours, and then
loaded with Apopxin Green Indicator for 30 minutes. The fluorescence intensity of Apopxin Green Indicator was measured with a
flow cytometer using FL1 channel.
ab176749 has been referenced in 47 publications.
- Zhang J et al. Elucidation of Proteus mirabilis as a Key Bacterium in Crohn's Disease Inflammation. Gastroenterology 160:317-330.e11 (2021). PubMed: 33011176
- Le TM et al. Ex vivo Induction of Apoptotic Mesenchymal Stem Cell by High Hydrostatic Pressure. Stem Cell Rev Rep 17:662-672 (2021). PubMed: 33128169
- Akimov MG et al. ACTH(6-9)PGP Peptide Protects SH-SY5Y Cells from H2O2, tert-Butyl Hydroperoxide, and Cyanide Cytotoxicity via Stimulation of Proliferation and Induction of Prosurvival-Related Genes. Molecules 26:N/A (2021). PubMed: 33810344
- Lu M et al. Sea Cucumber-Derived Peptides Alleviate Oxidative Stress in Neuroblastoma Cells and Improve Survival in C. elegans Exposed to Neurotoxic Paraquat. Oxid Med Cell Longev 2021:8842926 (2021). PubMed: 33959216
- Koh SS et al. Effect of Ergothioneine on 7-Ketocholesterol-Induced Endothelial Injury. Neuromolecular Med 23:184-198 (2021). PubMed: 33067719