Overview

  • Product name

    Apoptosis/ Necrosis Assay Kit (blue, red, green)
    See all Apoptosis/ Necrosis kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    1h 00m
  • Product overview

    Apoptosis/ Necrosis Assay Kit (blue, red, green) (ab176750) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.


    The PS sensor used in this kit has red fluorescence (Ex/Em = 630/660 nm) upon binding to membrane PS.


    Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.


    Loss of plasma membrane integrity, as demonstrated by the ability of the membrane-impermeable DNA Nuclear Green DCS1 dye (Ex/Em = 490/525 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.


    This apoptosis / necrosis assay also provides a live cell cytoplasm labeling dye CytoCalcein Violet 450 (Ex/Em = 405/450 nm) to label live cell cytoplasm.


    This kit is optimized to simultaneously detect cell apoptosis (Red), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.

  • Notes

    This product was previously called Apoptosis/ Necrosis Detection Kit.

    Other apoptosis assays

    For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

Images

  • The fluorescence image shows cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (red, stained by Apopxin Deep Red Indicator), and necrotic (green, indicated by Nuclear Green DCS1staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.

Protocols

References

This product has been referenced in:

  • Na M  et al. Both anti-TNF and CTLA4 Ig treatments attenuate the disease severity of staphylococcal dermatitis in mice. PLoS One 12:e0173492 (2017). Functional Studies . Read more (PubMed: 28264025) »
  • Nollet M  et al. A novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule. Oncotarget 8:112283-112296 (2017). Read more (PubMed: 29348825) »
See all 2 Publications for this product

Customer reviews and Q&As

Review for Apoptosis Necrosis/Detection Kit (ab176750)

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The fluorescence image indicates cells that are live (blue), apoptotic (red), and necrotic (green) in MEFs induced by 2 mM 3-MA for 3 hours. The fluorescence images of the cells were taken with a fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above.

Abcam user community

Verified customer

Submitted Jun 25 2018

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