Apoptosis/ Necrosis Detection Kit (blue, red, green) (ab176750)

Overview

  • Product name
    Apoptosis/ Necrosis Detection Kit (blue, red, green)
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    Apoptosis/ Necrosis Detection Kit (blue, red, green) (ab176750) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.

    The PS sensor used in this kit has red fluorescence (Ex/Em = 630/660 nm) upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable DNA Nuclear Green DCS1 (Ex/Em = 490/525 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis. In addition, this kit also provides a live cell cytoplasm labeling dye CytoCalcein Violet 450 (Ex/Em = 405/450 nm) for labeling live cell cytoplasm. This kit is optimized to simultaneously detect cell apoptosis (Red), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.

  • Notes

    Apoptosis is an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed.

  • Tested applications
    Suitable for: Flow Cyt, FMmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
FM Use at an assay dependent concentration.

Images

  • The fluorescence image shows cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (red, stained by Apopxin Deep Red Indicator), and necrotic (green, indicated by Nuclear Green DCS1staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.

Protocols

References

This product has been referenced in:
  • Na M  et al. Both anti-TNF and CTLA4 Ig treatments attenuate the disease severity of staphylococcal dermatitis in mice. PLoS One 12:e0173492 (2017). Functional Studies . Read more (PubMed: 28264025) »
  • Nollet M  et al. A novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule. Oncotarget 8:112283-112296 (2017). Read more (PubMed: 29348825) »

See all 2 Publications for this product

Customer reviews and Q&As

Review for Apoptosis Necrosis/Detection Kit (ab176750)

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The fluorescence image indicates cells that are live (blue), apoptotic (red), and necrotic (green) in MEFs induced by 2 mM 3-MA for 3 hours. The fluorescence images of the cells were taken with a fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above.
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Submitted Jun 25 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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