Apoptosis pathway knockout cell lines panel (ab275493)
Overview
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Product name
Apoptosis pathway knockout cell lines panel -
Product overview
A collection of 6 knockout cell lines involved in the apoptotic cell death pathways, in one kit for your convenience, with matching parental cell lines.
Find out more about our apoptosis assays.
Included in this panel:
FAS knockout HeLa cell Line (ab265260) + recommended control (ab255928)
FADD knockout HeLa cell Line (ab261817) + recommended control (ab255928)
DAXX knockout HeLa cell Line (ab265233) + recommended control (ab255928)
CASP8 knockout HeLa cell Line (ab264958) + recommended control (ab255448)
BAX knockout HeLa cell Line (ab255363) + recommended control (ab255448)
RIPK2 (RIP2) knockout HeLa cell Line (ab264688) + recommended control (ab255448)
Parts of the panels can be purchased separately. For further details please contact technical@abcam.com.
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Parental Cell Line
HeLa -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2 -
Passage number
<20 -
Knockout validation
Sanger Sequencing, Western Blot (WB) -
Notes
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
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Tested applications
Suitable for: WBmore details
Properties
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Components 1 kit ab255363 - Human BAX knockout HeLa cell line 1 x 1e+006cells/vial ab264958 - Human CASP8 knockout HeLa cell line 1 x 1e+006cells/vial ab265233 - Human DAXX knockout HeLa cell line 1 x 1e+006cells/vial ab261817 - Human FADD knockout HeLa cell line 1 x 1e+006cells/vial ab265260 - Human FAS knockout HeLa cell line 1 x 1e+006cells/vial ab264688 - Human RIPK2 knockout HeLa cell line 1 x 1e+006cells/vial ab255448 - Human Wild Type HeLa cell line 1 x 1e+006cells/vial ab255928 - Human Wild Type HeLa cell line 1 x 1e+006cells/vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female
Target
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Cellular localization
Bax: Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Daxx: Nucleus. Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1 and Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034). Colocalizes with RASSF1 in the nucleus (PubMed:18566590). Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383). Fas: Secreted and Cell membrane. RIP2: Cytoplasm. Caspase-8: Cytoplasm.
Associated products
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Assay kits
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KO cell lines
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KO cell lysates
- Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857)
- Human FAS knockout HeLa cell lysate (ab256911)
- Human FADD knockout HeLa cell lysate (ab257261)
- Human DAXX knockout HeLa cell lysate (ab257408)
- Human RIPK2 (RIP2) knockout HeLa cell lysate (ab258636)
- Human BAX knockout HeLa cell lysate (ab263841)
- Apoptosis pathway knockout cell lysates panel (ab275045)
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Related Products
- Anti-FADD antibody [EPR4415] (ab108601)
- Anti-FADD antibody [OTI1C11] (ab119059)
- Anti-Fas antibody [EPR5700] (ab133619)
- Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
- Anti-Bax antibody [EPR18284] (ab182734)
- Anti-Bax antibody [E63] - BSA and Azide free (ab216985)
- Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
- Anti-Daxx antibody [E94] - BSA and Azide free (ab239806)
- Anti-Bax antibody [EPR18284] - BSA and Azide free (ab250635)
- Anti-Caspase-8 antibody [E6] (ab32125)
- Anti-Daxx antibody [E94] (ab32140)
- Anti-Caspase-8 antibody [E7] (ab32397)
- Anti-Bax antibody [E63] (ab32503)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Images
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Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: CASP8 knockout HeLa cell lysate (20µg)
Lane 3: Jurkat cell lysate (20µg)
Lane 4: SH-SY5Y cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32125 Anti-Caspase-8 antibody [E6] was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 3000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: CASP8 knockout HeLa cell lysate (20µg)
Lane 3: Jurkat cell lysate (20µg)
Lane 4: SH-SY5Y cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab32397 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32397 Rabbit monoclonal [EPR2418Y] to IRF3 was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: FAS knockout HeLa cell lysate (20 µg)
Lanes 1-2: Merged signal (red and green). Green - ab133619 observed at 37 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133619 Anti-Fas antibody [EPR5700] was shown to specifically react with Fas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265260 (knockout cell lysate ab256911) was used. Wild-type and Fas knockout samples were subjected to SDS-PAGE. ab133619 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: FADD knockout HeLa cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108601 Anti-FADD antibody [EPR4415] was shown to specifically react with FADD in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261817 (knockout cell lysate ab257261) was used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: FADD knockout HeLa cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control ab181602 observed at 37 kDa.
ab119059 Anti-FADD antibody [OTI1C11] was shown to specifically react with FADD in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261817 (knockout cell lysate ab257261) was used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: DAXX knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab32140 observed at 100 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32140 Recombinant Anti-Daxx antibody [E94] was shown to specifically react with Daxx in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265233 (knockout cell lysate ab257408) was used. Wild-type and Daxx knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: BAX knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab182734 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.
ab182734 Recombinant Anti-Bax antibody [EPR18284 was shown to specifically react with BAX in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255363 (knockout cell lysate ab263841) was used. Wild-type and BAX knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab182734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: BAX knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab32503 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32503 Recombinant Anti-Bax antibody [E63] was shown to specifically react with BAX in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255363 (knockout cell lysate ab263841) was used. Wild-type and BAX knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: RIPK2 knockout HeLa cell lysate (20 µg)
Lane 3: Ramos cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab75257 observed at 65 kDa. Red - loading control ab181602 observed at 37 kDa.
ab75257 Anti-RIP2 antibody [AF28D3] was shown to specifically react with RIP2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264688 (knockout cell lysate ab258636) was used. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab75257 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275493 has not yet been referenced specifically in any publications.