Anti-NOL3 antibody (ab2002)
Key features and details
- Rabbit polyclonal to NOL3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOL3 antibody
See all NOL3 primary antibodies -
Description
Rabbit polyclonal to NOL3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NOL3 aa 191-208 (C terminal).
Sequence:PDPEPEPDFEERDESEDS
(Peptide available asab8371) -
Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | ||
WB |
Notes |
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WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Isoform 1 may be involved in RNA splicing.
Isoform 2 may inhibit apoptosis. -
Tissue specificity
Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas. -
Sequence similarities
Contains 1 CARD domain. -
Cellular localization
Cytoplasm and Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 8996 Human
- Omim: 605235 Human
- SwissProt: O60936 Human
- Unigene: 513667 Human
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Alternative names
- Apoptosis repressor with CARD antibody
- ARC antibody
- Muscle enriched cytoplasmic protein antibody
see all
Images
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All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : KB whole cell lysate
Lane 3 : A549 whole cell lysate
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.
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Immunohistochemistry of NOL3 in skeletal muscle with Anti-NOL3 (ab2002) at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab2002 has been referenced in 1 publication.
- Razorenova OV et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . PubMed: 24344197