Overview

  • Product name
    Anti-Apoptosis repressor with CARD antibody
    See all Apoptosis repressor with CARD primary antibodies
  • Description
    Rabbit polyclonal to Apoptosis repressor with CARD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Apoptosis repressor with CARD aa 191-208 (C terminal).
    Sequence:

    PDPEPEPDFEERDESEDS


    (Peptide available as ab8371)

  • Positive control
    • HeLa whole cell lysate
  • General notes
    Apoptosis Repressor with CARD domain

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Isoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.
    • Tissue specificity
      Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
    • Sequence similarities
      Contains 1 CARD domain.
    • Cellular localization
      Cytoplasm and Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis repressor with CARD antibody
      • ARC antibody
      • Muscle enriched cytoplasmic protein antibody
      • Muscle-enriched cytoplasmic protein antibody
      • MYC antibody
      • MYP antibody
      • Nol3 antibody
      • NOL3_HUMAN antibody
      • NOP antibody
      • Nop30 antibody
      • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
      • Nucleolar protein 3 antibody
      • Nucleolar protein of 30 kDa antibody
      see all

    Images

    • All lanes : Anti-Apoptosis repressor with CARD antibody (ab2002) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : KB whole cell lysate
      Lane 3 : A549 whole cell lysate

      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry of Apoptosis repressor with CARD in human heart tissue with Anti-Apoptosis repressor with CARD (ab2002) at 5 µg/ml.
    • Immunohistochemistry of Apoptosis repressor with CARD in skeletal muscle with Anti-Apoptosis repressor with CARD (ab2002) at 5 µg/ml.

    References

    This product has been referenced in:
    • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . Read more (PubMed: 24344197) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Q&A

    Answer

    Thank you for forwarding this enquiry.

    Reviewing the IHC images from the datasheet, I understood the customers concerns and contacted the testing laboratory.The images were provided tothem by a collaborator who uses red dyes for detection. In the human heart you can see darker pink/red areas of cytoplasmic staining. However, unfortunately it is difficult to tell with the skeletal muscle tissue. They are going to retest the antibody inIHC with standard DAB detection.

    I am sorry we are not able to offer anything further for this customer on this occasion as the antibody is already tested and covered by our guarantee in IHC-P. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet and the customer may like to consider this in return for Abpoint rewards. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contactme again with any other questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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