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We interpret the three FAS treatment specific bands in Jurkat cells as being various cleavage intermediates/products of the pro caspase 3 (uniprot P42574, http://www.uniprot.org/uniprot/P42574) processing. Specifically, amino acids 1-175 of P42574 predicted 19.8 kDa, amino acids 10-175 of P42574 predicted 18.7 kDa, amino acids 29-175 of P42574 predicted 16.6 kDa. As one can see from the figure, their relative ratio is changing with the time of the treatment. The pro/p17 caspase 3 antibody is a rabbit monoclonal antibody raised using immunogen within amino acids 50-150.
Elisa ThomasAbcam Scientific Support
Answered on Oct 21 2014