Product nameApoptotic Cell Enrichment Kit
Sample typeAdherent cells, Suspension cells
Assay time1h 00m
Abcam's Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while non-apoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
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Tested applicationsSuitable for: FM, Flow Cytmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 30 tests 1x Binding Buffer WM 1 x 25ml Annexin V-Biotin Yellow 1 x 150µl Apoptotic Cell Releasing Buffer NM 1 x 10ml Streptavidin-MagBeads Brown 1 x 1.5ml
RelevanceCell isolation is a way of dissociating cells from tissues and separating the various types in order to obtain a relatively homogeneous population of cells for analysis.
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Our Abpromise guarantee covers the use of ab65782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
ab65782 has not yet been referenced specifically in any publications.