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Apoptotic DNA Ladder Detection Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes, whereas other similar products in the market require 1-2 days. No DNA extraction or additional columns required.
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Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
|Ammonium Acetate Solution||Yellow||1 x 250µl|
|DNA Suspension Buffer||Green||1 x 1.5ml|
|Enzyme A Solution||Blue||1 x 250µl|
|Enzyme B (Lyophilized)||Red||1 vial|
|TE Lysis Buffer||Purple||1 x 1.8ml|
Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.
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