Anti-APP-1 antibody (ab220832)
Key features and details
- Rabbit polyclonal to APP-1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APP-1 antibody
See all APP-1 primary antibodies -
Description
Rabbit polyclonal to APP-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human APP-1 aa 350-450.
Database link: Q13310 -
Positive control
- WB: U-2197 and HeLa cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 71 kDa.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| Notes |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 71 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. -
Tissue specificity
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated. -
Sequence similarities
Belongs to the polyadenylate-binding protein type-1 family.
Contains 1 PABC domain.
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-518 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 8761 Human
- Omim: 603407 Human
- SwissProt: Q13310 Human
- Unigene: 169900 Human
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Alternative names
- Activated platelet protein 1 antibody
- Activated-platelet protein 1 antibody
- APP 1 antibody
see all
Images
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Western blot - Anti-APP-1 antibody (ab220832)All lanes : Anti-APP-1 antibody (ab220832) at 0.4 µg/ml
Lane 1 : U-2197 cell line
Lane 2 : HeLa cell line
Predicted band size: 71 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-APP-1 antibody (ab220832)Immunofluorescent analysis of PFA fixed, triton X-100 permeabilized U-2 OS cells labeling APP-1 with ab220832 at 4 µg/mL (green).
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Western blot - Anti-APP-1 antibody (ab220832)All lanes : Anti-APP-1 antibody (ab220832) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 71 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab220832 has been referenced in 1 publication.
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708