Product nameAnti-APP-1 antibody
See all APP-1 primary antibodies
DescriptionRabbit polyclonal to APP-1
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human APP-1 aa 594-644. The exact sequence is proprietary. NP_003810.1
Database link: Q13310
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: APP-1 IP in HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to APP-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
|WB||1/2000 - 1/10000.|
FunctionBinds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.
Tissue specificityExpressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
Sequence similaritiesBelongs to the polyadenylate-binding protein type-1 family.
Contains 1 PABC domain.
Contains 4 RRM (RNA recognition motif) domains.
modificationsArg-518 is dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- Activated platelet protein 1 antibody
- Activated-platelet protein 1 antibody
- APP 1 antibody
All lanes : Anti-APP-1 antibody (ab241592) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 seconds
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for APP-1 using ab241592 at 1/200 dilution in immunohistochemical analysis.
APP-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (mg for IP, 20% of IP loaded) using ab241592 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241592 at 1 µg/mL.
Lane 1: ab241592 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
ab241592 has not yet been referenced specifically in any publications.