The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
1/50 - 1/100.
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Ubiquitous in fetal tissues. Expressed throughout the adult brain.
Protein modification; protein neddylation.
Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.
Cell membrane. Colocalizes with APP in lipid rafts.
ab71526, at 1/50 dilution, staining APPBP1 in human breast carcinoma by immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by a peroxidase-conjugated secondary antibody and DAB staining.