Recombinant
RabMAb

Recombinant Anti-APPBP1 antibody [EPR15732] (ab187142)

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Overview

  • Product name

    Anti-APPBP1 antibody [EPR15732]
    See all APPBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15732] to APPBP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human APPBP1 aa 50-150. The exact sequence is proprietary.
    Database link: Q13564

  • Positive control

    • HeLa, A431, Human fetal muscle, Rat brain, Rat heart, Rat spleen and Human fetal brain lysates; HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/30 - 1/40.

Target

  • Function

    Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

  • Tissue specificity

    Ubiquitous in fetal tissues. Expressed throughout the adult brain.

  • Pathway

    Protein modification; protein neddylation.

  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

  • Cellular localization

    Cell membrane. Colocalizes with APP in lipid rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • 59 kDa antibody
      • A 116A10.1 antibody
      • Amyloid beta precursor protein binding protein 1 59kD antibody
      • Amyloid beta precursor protein binding protein 1 59kDa antibody
      • Amyloid beta precursor protein binding protein 1 antibody
      • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
      • Amyloid beta precursor protein-binding protein 1 antibody
      • Amyloid protein binding protein 1 antibody
      • Amyloid protein-binding protein 1 antibody
      • APP BP1 antibody
      • APP-BP1 antibody
      • APPBP 1 antibody
      • APPBP1 antibody
      • HPP 1 antibody
      • HPP1 antibody
      • NAE 1 antibody
      • NAE1 antibody
      • NEDD8 activating enzyme E1 regulatory subunit antibody
      • NEDD8 activating enzyme E1 subunit 1 antibody
      • NEDD8 activating enzyme E1 subunit antibody
      • NEDD8-activating enzyme E1 regulatory subunit antibody
      • Proto oncogene protein 1 antibody
      • Proto-oncogene protein 1 antibody
      • Protooncogene protein 1 antibody
      • ula 1 antibody
      • ULA1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Human fetal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 60 kDa

    • Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)

      Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)  labeling APPBP1 with ab187142 at 1/120 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    • Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)

      Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab187142 at 1/50 dilution (lane 1) or PBS control (lane 2).
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat heart tissue lysate
      Lane 3 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 60 kDa

    References

    ab187142 has not yet been referenced specifically in any publications.

    Publishing research using ab187142? Please let us know so that we can cite the reference in this datasheet.

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