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    appbp1-antibody-epr15732-ab187142.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins
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RecombinantRabMAb

Recombinant Anti-APPBP1 antibody [EPR15732] (ab187142)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Abpromise

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Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
  • Anti-APPBP1 antibody [EPR15732] (ab187142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15732] to APPBP1
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-APPBP1 antibody [EPR15732]
    See all APPBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15732] to APPBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human APPBP1 aa 50-150. The exact sequence is proprietary.
    Database link: Q13564

  • Positive control

    • HeLa, A431, Human fetal muscle, Rat brain, Rat heart, Rat spleen and Human fetal brain lysates; HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15732
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Associated products

  • Alternative Versions

    • Anti-APPBP1 antibody [EPR15732] - BSA and Azide free (ab250906)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human APPBP1 protein (ab116199)
  • Related Products

    • Recombinant Human APPBP1 protein (ab116199)

Applications

Our Abpromise guarantee covers the use of ab187142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30 - 1/40.

Target

  • Function

    Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
  • Tissue specificity

    Ubiquitous in fetal tissues. Expressed throughout the adult brain.
  • Pathway

    Protein modification; protein neddylation.
  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.
  • Cellular localization

    Cell membrane. Colocalizes with APP in lipid rafts.
  • Target information above from: UniProt accession Q13564 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8883 Human
    • Entrez Gene: 84019 Rat
    • Omim: 603385 Human
    • SwissProt: Q13564 Human
    • SwissProt: Q9Z1A5 Rat
    • Unigene: 460978 Human
    • Unigene: 4279 Rat
    • Alternative names

      • 59 kDa antibody
      • A 116A10.1 antibody
      • Amyloid beta precursor protein binding protein 1 59kD antibody
      • Amyloid beta precursor protein binding protein 1 59kDa antibody
      • Amyloid beta precursor protein binding protein 1 antibody
      • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
      • Amyloid beta precursor protein-binding protein 1 antibody
      • Amyloid protein binding protein 1 antibody
      • Amyloid protein-binding protein 1 antibody
      • APP BP1 antibody
      • APP-BP1 antibody
      • APPBP 1 antibody
      • APPBP1 antibody
      • HPP 1 antibody
      • HPP1 antibody
      • NAE 1 antibody
      • NAE1 antibody
      • NEDD8 activating enzyme E1 regulatory subunit antibody
      • NEDD8 activating enzyme E1 subunit 1 antibody
      • NEDD8 activating enzyme E1 subunit antibody
      • NEDD8-activating enzyme E1 regulatory subunit antibody
      • Proto oncogene protein 1 antibody
      • Proto-oncogene protein 1 antibody
      • Protooncogene protein 1 antibody
      • ula 1 antibody
      • ULA1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Human fetal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 60 kDa

    • Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Flow Cytometry - Anti-APPBP1 antibody [EPR15732] (ab187142)

      Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)  labeling APPBP1 with ab187142 at 1/120 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    • Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Immunoprecipitation - Anti-APPBP1 antibody [EPR15732] (ab187142)

      Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab187142 at 1/50 dilution (lane 1) or PBS control (lane 2).
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      Western blot - Anti-APPBP1 antibody [EPR15732] (ab187142)
      All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat heart tissue lysate
      Lane 3 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 60 kDa

    • Anti-APPBP1 antibody [EPR15732] (ab187142)
      Anti-APPBP1 antibody [EPR15732] (ab187142)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab187142? Please let us know so that we can cite the reference in this datasheet.

    ab187142 has been referenced in 1 publication.

    • Zeng Y  et al. An overactive neddylation pathway serves as a therapeutic target and MLN4924 enhances the anticancer activity of cisplatin in pancreatic cancer. Oncol Lett 18:2724-2732 (2019). PubMed: 31404297

    Customer reviews and Q&As

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