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Rabbit polyclonal to APPL
Mouse, Rat, Hamster, Human, Monkey
Recombinant full length protein (Human) expressed in E.coli
HEK293 cell lysate
2C 12 cells
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
Constituents: 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).
Use at an assay dependent dilution.
Use a concentration of 0.5 - 1 µg/ml.
Use a concentration of 1 - 2 µg/ml.
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
High levels in heart, ovary, pancreas and skeletal muscle.
Contains 1 PH domain.
Contains 1 PID domain.
Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Information by UniProt
Adapter protein containing PH domain antibody
Adaptor Protein Containing pH Domain antibody
Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
Western blot - Anti-APPL antibody (ab95195)
All lanes : Anti-APPL antibody (ab95195) at 0.2 µg/ml Lane 1 : HEK293 cell lysate Lane 2 : C 2C 12 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat anti-rabbit antibody Predicted band size: 80 kDa
Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody (ab95195)
Immunostaining of APPL in C
2C 12 cells using ab95195 at 2 µg/ml followed by goat anti rabbit antibody, visualized by confocal microscopy.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"