Key features and details
- Rabbit polyclonal to APPL
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG
Product nameAnti-APPL antibody
See all APPL primary antibodies
DescriptionRabbit polyclonal to APPL
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Recombinant full length protein (Human) expressed in E.coli
Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab95195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
FunctionRequired for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
Tissue specificityHigh levels in heart, ovary, pancreas and skeletal muscle.
Sequence similaritiesContains 1 PH domain.
Contains 1 PID domain.
DomainOverexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationEarly endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
- Information by UniProt
- Adapter protein containing PH domain antibody
- Adaptor Protein Containing pH Domain antibody
- Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
All lanes : Anti-APPL antibody (ab95195) at 0.2 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat
Predicted band size: 80 kDa
Immunostaining of APPL in C2C12 cells using ab95195 at 2 µg/ml followed by goat anti rabbit antibody, visualized by confocal microscopy.
ab95195 has been referenced in 1 publication.
- Jiang G et al. Mediation of insulin growth factor-1 in Alzheimer's disease and the mechanism of PRNP genetic expression and the PI3K/Akt signaling pathway. Exp Ther Med 13:2763-2766 (2017). WB . PubMed: 28587338