Recombinant
RabMAb

Recombinant Anti-APPL antibody [EPR13569] (ab180140)

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Overview

  • Product name

    Anti-APPL antibody [EPR13569]
    See all APPL primary antibodies

  • Description

    Rabbit monoclonal [EPR13569] to APPL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human APPL aa 400-500 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UKG1

  • Positive control

    • HeLa, MCF7 and 293 lysates. Human colonic carcinoma and Human pancreas tissue. MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab180140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
ICC/IF 1/250.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.

  • Tissue specificity

    High levels in heart, ovary, pancreas and skeletal muscle.

  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 PID domain.

  • Domain

    Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adapter protein containing PH domain antibody
      • Adaptor Protein Containing pH Domain antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
      • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
      • AKT2 INTERACTOR antibody
      • APPL 1 antibody
      • Appl1 antibody
      • DCC interacting protein 13 alpha antibody
      • DCC-interacting protein 13-alpha antibody
      • DIP 13 alpha antibody
      • DIP13 alpha antibody
      • Dip13-alpha antibody
      • DIP13alpha antibody
      • DP13A_HUMAN antibody
      • KIAA1428 antibody
      • PTB domain and leucine zipper motif 1 antibody
      • PTB Domain and Leucine Zipper Motif antibody
      • Signaling adaptor protein DIP13alpha antibody
      see all

    Images

    • Western blot - Anti-APPL antibody [EPR13569] (ab180140)
      Western blot - Anti-APPL antibody [EPR13569] (ab180140)
      All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 80 kDa
      Observed band size: 90 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)
      Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)

      Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling APPL with ab180140 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 555) Conterstained with Dapi blue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)

      Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)

      Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Li W  et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). Read more (PubMed: 29335016) »
    • Wang T  et al. Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity. Sci Rep 7:7497 (2017). Read more (PubMed: 28790390) »
    See all 4 Publications for this product

    Publishing research using ab180140? Please let us know so that we can cite the reference in this datasheet.

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