Overview

  • Product name
    Anti-APPL antibody [EPR13569]
    See all APPL primary antibodies
  • Description
    Rabbit monoclonal [EPR13569] to APPL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human APPL aa 400-500 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UKG1

  • Positive control
    • HeLa, MCF7 and 293 lysates. Human colonic carcinoma and Human pancreas tissue. MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
ICC/IF 1/250.
IHC-P 1/100.

Target

  • Function
    Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
  • Tissue specificity
    High levels in heart, ovary, pancreas and skeletal muscle.
  • Sequence similarities
    Contains 1 PH domain.
    Contains 1 PID domain.
  • Domain
    Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter protein containing PH domain antibody
    • Adaptor Protein Containing pH Domain antibody
    • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
    • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
    • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
    • AKT2 INTERACTOR antibody
    • APPL 1 antibody
    • Appl1 antibody
    • DCC interacting protein 13 alpha antibody
    • DCC-interacting protein 13-alpha antibody
    • DIP 13 alpha antibody
    • DIP13 alpha antibody
    • Dip13-alpha antibody
    • DIP13alpha antibody
    • DP13A_HUMAN antibody
    • KIAA1428 antibody
    • PTB domain and leucine zipper motif 1 antibody
    • PTB Domain and Leucine Zipper Motif antibody
    • Signaling adaptor protein DIP13alpha antibody
    see all

Images

  • All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling APPL with ab180140 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 555) Conterstained with Dapi blue.

  • Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

References

This product has been referenced in:
  • Li W  et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). Read more (PubMed: 29335016) »
  • Wang T  et al. Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity. Sci Rep 7:7497 (2017). Read more (PubMed: 28790390) »
See all 4 Publications for this product

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