Recombinant Anti-APPL antibody [EPR13569] (ab180140)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-APPL antibody [EPR13569]
See all APPL primary antibodies -
DescriptionRabbit monoclonal [EPR13569] to APPL
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human APPL aa 400-500 (internal sequence). The exact sequence is proprietary.
Database link: Q9UKG1 -
Positive control
- HeLa, MCF7 and 293 lysates. Human colonic carcinoma and Human pancreas tissue. MCF7 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13569
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab180140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa). | |
| ICC/IF | 1/250. | |
| IHC-P | 1/100. |
Target
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FunctionRequired for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
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Tissue specificityHigh levels in heart, ovary, pancreas and skeletal muscle.
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Sequence similaritiesContains 1 PH domain.
Contains 1 PID domain. -
DomainOverexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationEarly endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
- Information by UniProt
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Database links
- Entrez Gene: 26060 Human
- Entrez Gene: 72993 Mouse
- Entrez Gene: 290537 Rat
- Omim: 604299 Human
- SwissProt: Q9UKG1 Human
- SwissProt: Q8K3H0 Mouse
- Unigene: 476415 Human
- Unigene: 202322 Mouse
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Alternative names
- Adapter protein containing PH domain antibody
- Adaptor Protein Containing pH Domain antibody
- Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
see all
Images
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![Western blot - Anti-APPL antibody [EPR13569] (ab180140)](https://www.abcam.com/ps/products/180/ab180140/Images/ab180140-210015-ab180140.jpg)
Western blot - Anti-APPL antibody [EPR13569] (ab180140)All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)](https://www.abcam.com/ps/products/180/ab180140/Images/ab180140-210018-ab1801404.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling APPL with ab180140 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 555) Conterstained with Dapi blue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)](https://www.abcam.com/ps/products/180/ab180140/Images/ab180140-210017-ab1801403.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)](https://www.abcam.com/ps/products/180/ab180140/Images/ab180140-210016-ab1801402.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Li W et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). Read more (PubMed: 29335016) »
- Wang T et al. Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity. Sci Rep 7:7497 (2017). Read more (PubMed: 28790390) »
