RecombinantRabMAb

Recombinant Anti-APPL antibody [EPR13569] (ab180140)

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Western blot - Anti-APPL antibody [EPR13569] (ab180140)
  • Western blot - Anti-APPL antibody [EPR13569] (ab180140)
  • Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
  • Anti-APPL antibody [EPR13569] (ab180140)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13569] to APPL
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-APPL antibody [EPR13569]
    See all APPL primary antibodies

  • Description

    Rabbit monoclonal [EPR13569] to APPL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human APPL aa 400-500 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UKG1

  • Positive control

    • WB: HeLa, MCF7 and 293 lysates. IHC-P: Human colonic carcinoma and Human pancreas tissue. ICC: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
ICC/IF
1/250.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
ICC/IF
1/250.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.

  • Tissue specificity

    High levels in heart, ovary, pancreas and skeletal muscle.

  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 PID domain.

  • Domain

    Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adapter protein containing PH domain antibody
      • Adaptor Protein Containing pH Domain antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
      • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
      • AKT2 INTERACTOR antibody
      • APPL 1 antibody
      • Appl1 antibody
      • DCC interacting protein 13 alpha antibody
      • DCC-interacting protein 13-alpha antibody
      • DIP 13 alpha antibody
      • DIP13 alpha antibody
      • Dip13-alpha antibody
      • DIP13alpha antibody
      • DP13A_HUMAN antibody
      • KIAA1428 antibody
      • PTB domain and leucine zipper motif 1 antibody
      • PTB Domain and Leucine Zipper Motif antibody
      • Signaling adaptor protein DIP13alpha antibody
      see all

    Images

    • Western blot - Anti-APPL antibody [EPR13569] (ab180140)
      Western blot - Anti-APPL antibody [EPR13569] (ab180140)
      All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : APPL CRISPR/Cas9 edited HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HEK-293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 80 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      Lanes 1- 4: Merged signal (red and green). Green - ab180140 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab180140 was shown to react with Anti-APPL in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab265187 (CRISPR/Cas9 edited cell lysate ab257836) lane below 90kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and APPL1 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-APPL antibody [EPR13569] (ab180140)
      Western blot - Anti-APPL antibody [EPR13569] (ab180140)
      All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 80 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)
      Immunocytochemistry/ Immunofluorescence - Anti-APPL antibody [EPR13569] (ab180140)

      Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling APPL with ab180140 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 555) Conterstained with Dapi blue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)

      Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] (ab180140)

      Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-APPL antibody [EPR13569] (ab180140)
      Anti-APPL antibody [EPR13569] (ab180140)

    References (5)

    Publishing research using ab180140? Please let us know so that we can cite the reference in this datasheet.

    ab180140 has been referenced in 5 publications.

    • Li W  et al. Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model. Stem Cell Res Ther 9:9 (2018). PubMed: 29335016
    • Wang T  et al. Involvement of Insulin Signaling Disturbances in Bisphenol A-Induced Alzheimer's Disease-like Neurotoxicity. Sci Rep 7:7497 (2017). PubMed: 28790390
    • Zhen YB  et al. Expression of TAG1/APP signaling pathway in the proliferation and differentiation of glioma stem cells. Oncol Lett 14:2137-2140 (2017). PubMed: 28789439
    • Ma J  et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441
    • Yoon HY  et al. ARAP1 regulates endocytosis of EGFR. Traffic 9:2236-52 (2008). PubMed: 18939958

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