Key features and details
- Rabbit polyclonal to APPL - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-APPL antibody - N-terminal
See all APPL primary antibodies
DescriptionRabbit polyclonal to APPL - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Pig, Xenopus laevis, Zebrafish
Recombinant fragment, corresponding to a region within N-terminal amino acids 1-205 of Human APPL (UniProt: Q9UKG1).
- U-87 MG and MCF7 whole cell lysates; Human AZ521 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 80 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
Tissue specificityHigh levels in heart, ovary, pancreas and skeletal muscle.
Sequence similaritiesContains 1 PH domain.
Contains 1 PID domain.
DomainOverexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationEarly endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
- Information by UniProt
- Adapter protein containing PH domain antibody
- Adaptor Protein Containing pH Domain antibody
- Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
ab153809 has been referenced in 1 publication.
- Galan-Davila AK et al. Alternative splicing variant of the scaffold protein APPL1 suppresses hepatic adiponectin signaling and function. J Biol Chem 293:6064-6074 (2018). PubMed: 29483192