Anti-APPL antibody - N-terminal (ab226185)

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Overview

  • Product name

    Anti-APPL antibody - N-terminal
    See all APPL primary antibodies

  • Description

    Rabbit polyclonal to APPL - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide within Human APPL aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: Q9UKG1-1

  • Positive control

    • IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.

    • Tissue specificity

      High levels in heart, ovary, pancreas and skeletal muscle.

    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 PID domain.

    • Domain

      Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adapter protein containing PH domain antibody
      • Adaptor Protein Containing pH Domain antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
      • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
      • AKT2 INTERACTOR antibody
      • APPL 1 antibody
      • Appl1 antibody
      • DCC interacting protein 13 alpha antibody
      • DCC-interacting protein 13-alpha antibody
      • DIP 13 alpha antibody
      • DIP13 alpha antibody
      • Dip13-alpha antibody
      • DIP13alpha antibody
      • DP13A_HUMAN antibody
      • KIAA1428 antibody
      • PTB domain and leucine zipper motif 1 antibody
      • PTB Domain and Leucine Zipper Motif antibody
      • Signaling adaptor protein DIP13alpha antibody
      see all

    Images

    • Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)
      Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)

      APPL was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226185 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226185 at 0.4 µg/ml.

      Lane 1: ab226185 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    References

    ab226185 has not yet been referenced specifically in any publications.

    Publishing research using ab226185? Please let us know so that we can cite the reference in this datasheet.

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