Overview

  • Product name
    Anti-APPL antibody - N-terminal
    See all APPL primary antibodies
  • Description
    Rabbit polyclonal to APPL - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human APPL aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: Q9UKG1-1

  • Positive control
    • IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
    • Tissue specificity
      High levels in heart, ovary, pancreas and skeletal muscle.
    • Sequence similarities
      Contains 1 PH domain.
      Contains 1 PID domain.
    • Domain
      Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter protein containing PH domain antibody
      • Adaptor Protein Containing pH Domain antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
      • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
      • AKT2 INTERACTOR antibody
      • APPL 1 antibody
      • Appl1 antibody
      • DCC interacting protein 13 alpha antibody
      • DCC-interacting protein 13-alpha antibody
      • DIP 13 alpha antibody
      • DIP13 alpha antibody
      • Dip13-alpha antibody
      • DIP13alpha antibody
      • DP13A_HUMAN antibody
      • KIAA1428 antibody
      • PTB domain and leucine zipper motif 1 antibody
      • PTB Domain and Leucine Zipper Motif antibody
      • Signaling adaptor protein DIP13alpha antibody
      see all

    Images

    • APPL was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226185 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226185 at 0.4 µg/ml.

      Lane 1: ab226185 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    References

    ab226185 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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