- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-APPL antibody - N-terminal
See all APPL primary antibodies -
DescriptionRabbit polyclonal to APPL - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: IPmore details
Unsuitable for: WB -
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human APPL aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9UKG1-1 -
Positive control
- IP: HEK-293T whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab226185 was affinity purified using an epitope specific to APPL immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab226185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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FunctionRequired for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
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Tissue specificityHigh levels in heart, ovary, pancreas and skeletal muscle.
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Sequence similaritiesContains 1 PH domain.
Contains 1 PID domain. -
DomainOverexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationEarly endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
- Information by UniProt
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Database links
- Entrez Gene: 101096960 Cat
- Entrez Gene: 460469 Chimpanzee
- Entrez Gene: 517850 Cow
- Entrez Gene: 484716 Dog
- Entrez Gene: 101126724 Gorilla
- Entrez Gene: 26060 Human
- Entrez Gene: 72993 Mouse
- Entrez Gene: 100442490 Orangutan
see all -
Alternative names
- Adapter protein containing PH domain antibody
- Adaptor Protein Containing pH Domain antibody
- Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
see all
Images
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Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)APPL was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226185 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226185 at 0.4 µg/ml.
Lane 1: ab226185 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
References
ab226185 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"