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    appl-antibody-n-terminal-ab226185.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-APPL antibody - N-terminal (ab226185)

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Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)

    Key features and details

    • Rabbit polyclonal to APPL - N-terminal
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-APPL antibody - N-terminal
      See all APPL primary antibodies
    • Description

      Rabbit polyclonal to APPL - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide within Human APPL aa 1-50 (N terminal). The exact sequence is proprietary.
      Database link: Q9UKG1-1

    • Positive control

      • IP: HEK-293T whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab226185 was affinity purified using an epitope specific to APPL immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Associated Proteins
      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Signal Transduction
      • Protein Trafficking
      • Organelle Proteins
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Developmental Biology
      • Organogenesis
      • Nervous system development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab226185 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at 2-10 µg/mg of lysate.
    Notes
    IP
    Use at 2-10 µg/mg of lysate.

    Target

    • Function

      Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
    • Tissue specificity

      High levels in heart, ovary, pancreas and skeletal muscle.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 PID domain.
    • Domain

      Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
    • Target information above from: UniProt accession Q9UKG1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 101096960 Cat
      • Entrez Gene: 460469 Chimpanzee
      • Entrez Gene: 517850 Cow
      • Entrez Gene: 484716 Dog
      • Entrez Gene: 101126724 Gorilla
      • Entrez Gene: 26060 Human
      • Entrez Gene: 72993 Mouse
      • Entrez Gene: 100442490 Orangutan
      • Entrez Gene: 100188978 Pig
      • Entrez Gene: 100353097 Rabbit
      • Entrez Gene: 100425753 Rhesus monkey
      • Omim: 604299 Human
      • SwissProt: Q9UKG1 Human
      • SwissProt: Q8K3H0 Mouse
      • Unigene: 476415 Human
      • Unigene: 202322 Mouse
      see all
    • Alternative names

      • Adapter protein containing PH domain antibody
      • Adaptor Protein Containing pH Domain antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
      • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
      • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
      • AKT2 INTERACTOR antibody
      • APPL 1 antibody
      • Appl1 antibody
      • DCC interacting protein 13 alpha antibody
      • DCC-interacting protein 13-alpha antibody
      • DIP 13 alpha antibody
      • DIP13 alpha antibody
      • Dip13-alpha antibody
      • DIP13alpha antibody
      • DP13A_HUMAN antibody
      • KIAA1428 antibody
      • PTB domain and leucine zipper motif 1 antibody
      • PTB Domain and Leucine Zipper Motif antibody
      • Signaling adaptor protein DIP13alpha antibody
      see all

    Images

    • Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)
      Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)

      APPL was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226185 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226185 at 0.4 µg/ml.

      Lane 1: ab226185 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab226185? Please let us know so that we can cite the reference in this datasheet.

    ab226185 has not yet been referenced specifically in any publications.

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