Anti-APPL antibody - N-terminal (ab226185)
Key features and details
- Rabbit polyclonal to APPL - N-terminal
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APPL antibody - N-terminal
See all APPL primary antibodies -
Description
Rabbit polyclonal to APPL - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human APPL aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9UKG1-1 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226185 was affinity purified using an epitope specific to APPL immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. -
Tissue specificity
High levels in heart, ovary, pancreas and skeletal muscle. -
Sequence similarities
Contains 1 PH domain.
Contains 1 PID domain. -
Domain
Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. - Information by UniProt
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Database links
- Entrez Gene: 101096960 Cat
- Entrez Gene: 460469 Chimpanzee
- Entrez Gene: 517850 Cow
- Entrez Gene: 484716 Dog
- Entrez Gene: 101126724 Gorilla
- Entrez Gene: 26060 Human
- Entrez Gene: 72993 Mouse
- Entrez Gene: 100442490 Orangutan
see all -
Alternative names
- Adapter protein containing PH domain antibody
- Adaptor Protein Containing pH Domain antibody
- Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
see all
Images
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Immunoprecipitation - Anti-APPL antibody - N-terminal (ab226185)
APPL was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226185 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226185 at 0.4 µg/ml.
Lane 1: ab226185 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226185 has not yet been referenced specifically in any publications.